系统性红斑狼疮患者外周血淋巴细胞凋亡及其临床意义  

Prliminary Study on Apoptosis in Peripheral Blood Lymphocytes ofPatients with Systemic Lupus Erythematosus

作　　者：谢敏[1] 王曾礼[1] 刘小菁[1] 强欧[1] 刘刚[1] 

机构地区：[1]华西医科大学附属第一医院内科

出　　处：《华西医学》1999年第2期137-139,共3页West China Medical Journal

摘　　要：目的：通过２９例系统性红斑狼疮（ＳＬＥ）外周血淋巴细胞的凋亡现象观察，探讨细胞凋亡及其相关基因与ＳＬＥ病情的关系。方法：采用末端脱氧核苷酸转移酶介导的缺口末端标记（ＴＵＮＥＬ）法及流式细胞仪（ＦＣＭ）观察ＳＬＥ外周血淋巴细胞凋亡现象。结果：①ＳＬＥ患者外周血淋巴细胞凋亡率明显高于正常组及类风湿关节炎（ＲＡ）组（Ｐ＜０００１），经４８小时培养后，凋亡率更高可达３３４４％；②凋亡率与疾病活动程度呈正相关（ｒ＝０８６６），Ｐ＜０００１）。Objective:To observe the relationship between systemic lupus erythematosus(SLE)peripheral blood lymphocytes(PBL)apoptosis and its clinical significance.Methods:Terminal deoxynucleotidyl transferase mediated nick end labelling(TUNEL)technique and flow cytometry(FCM)were used to detect the apoptotic cell in 29 cases of SLE.Results:The rate of apoptosis of PBL was significantly higher in SLE patients than those in normal control(P<0001)and in rheumatoid arthritis(RA)(P<0001),After 48 hour culture the apoptotic rate was further increased in SLE patients(334%).There was a significant correlation between SLE activity and rate of apoptosis in vitro(r=0866,P<0001).Conclusion:Increased rate of apoptosis may be an index of valuation of severity of SLE patients.

关 键 词：系统性红斑狼疮 细胞凋亡 淋巴细胞 

分 类 号：R593.241[医药卫生—内科学]