生物心瓣材料细胞毒性试验方法敏感性比较  

Comparison of Sensitivity of Different Testing Methods in Assessing theCytotoxicity of Bioprosthetic Heart Valve Materials

作　　者：程述森[1,2] 石应康[1,2] 梁卫东[1,2] 李炜如[1,2] 熊英 

机构地区：[1]华西医科大学附属第一医院胸心外科 [2]华西医科大学附属第二医院儿科

出　　处：《华西医学》1999年第2期155-157,共3页West China Medical Journal

基　　金：国家自然科学基金

摘　　要：此研究采用培养的人胚肺成纤维细胞（ＨＥＬ）和鼠Ｌ－９２９细胞、分别采用琼脂覆盖法和直接接触法对商业用生物心瓣材料的细胞毒性分析比较。计算浸泡前和浸泡１０、２０、３０天周期中材料的细胞增殖抑制指数，并参照国际标准化组织（ＩＳＯ）要求评定材料细胞毒性。结果显示：在琼脂覆盖法试验中，无论使用ＨＥＬ或Ｌ－９２９细胞在生物心瓣材料各浸泡阶段均未观察到明显的脱色区和细胞溶解现象，细胞毒性评价为０－Ⅰ级。在直接接触法试验中，ＨＥＬ或Ｌ－９２９细胞在生物心瓣材料各浸泡阶段均显示出明显长期的细胞毒性。作者认为，商业用生物心瓣材料的细胞毒性试验使用直接接触法更为敏感与准确。Objective:The difference in cytotoxicity of bioprosthetic cardiac valve materials was compared by using agar overlay and direct contact assay methods.Method:The material was crosslinked by glutaral dehyde and stored in 4% formaldehyde.The cells used for testing were human embryonic fibroblasts and mouse L-929 cells.The agar overlay and direct contact assay methods were used and their results were analyzed and compared.The cell proliferative inhibition index(CPII)was calculated for the testing materials being at the periods before,10,20 and 30 days after immerising the testing materials in the cellular suspension fluid.Conclusion:It was concluded that the direct contact method was more sensitive in the detection of cytotoxicity of the material than that of the agar overlay method.It was considered that in testing the cytotoxicity of the prosthetic heart valve material the direct contact method was preferable because it was more sensitive and accurate.

关 键 词：生物心脏瓣膜 细胞毒性 试验方法 敏感性 

分 类 号：R318.11[医药卫生—生物医学工程]