生物体扫描图象的“仿荧光”三维重建  

THREE DIMENSIONAL SIMULATED FLUORESCENCE RECONSTRUCTION ALGORITHM FOR SCANNING BIOLOGICAL IMAGES

作　　者：向际鹰[1] 吴震[1] 骆清铭[1] 曾绍群[1] 张平[1] 黄德修[1] 

机构地区：[1]华中理工大学光电子系

出　　处：《中国生物医学工程学报》1999年第2期165-170,共6页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金

摘　　要：提出一种针对生物体共焦层析图象的三维重建算法，在荧光激发数学模型的基础上研究定向光线在空间中的传输，逐层递推得到任意方向上的消隐视图。该算法可较好重现试样的半透明效果，根据需要调整参数可使同一试样呈现透明、半透明或不透明的外观，辅助定向光源的引入有助于突出深层组织的细节结构，且具有其它算法无法比拟的运算速度。给出了算法的实现及实例。A so called simulated fluorescence sectional reconstruction method especially suitable for three dimensional biological confocal images is proposed. Transformation of directional illumination is investigated by ways of fluorescence stimulate. The algorithm is repeated layer by layer so as to render the views in arbitrary path, semitransparent area of biological samples can be well processed. Transparent, semi transparent or opacity outlook of the same object are available simultaneity by simple adjustment of several parameters. The details originally hide in deep layers can be shown with the aid of auxiliary directional source. It can provide far more acceptable speed compared with conventional methods as well. The realization and examples of the algorithm are also given.

关 键 词：共焦扫描成象 三维重建 荧光 生物体 

分 类 号：R318.03[医药卫生—生物医学工程]