神经细胞凋亡与胞浆钙离子  

Neuronal apoptosis and cytoplasmic calcium

作　　者：韩恩吉[1,2] 韩淑英 

机构地区：[1]济南山东医科大学附属医院神经内科 [2]山东省淄博市周村第二人民医院

出　　处：《中风与神经疾病杂志》1999年第3期131-132,共2页Journal of Apoplexy and Nervous Diseases

基　　金：世界卫生组织(WHO)国际神经科学研究基金

摘　　要：目的应用钙载体和ＰＣ１２细胞株探讨胞浆钙离子与神经细胞凋亡之间的关系。方法水母发光蛋白导入法测量ＰＣ１２胞浆钙离子，细胞存活率监测细胞死亡，ＤＮＡ琼脂糖电泳证实细胞凋亡。结果钙载体（Ａ２３１８７）升高ＰＣ１２胞浆钙离子，降低细胞存活率，ＤＮＡ琼脂糖电泳显示典型的ＤＮＡ梯形带。结论胞浆钙离子升高激活内源性核酸内切酶，导致ＤＮＡ核小体间断裂，启动神经细胞凋亡。Objective Relationship between neuronal apoptosis and changes in cytoplasmic calcium was studied using A23187,calcium ionophore,and PC12 cell line. Methods Changes in cytoplasmic calcium was measured with aequorinloaded method, neuronal death was shown in cell viability,DNA fragmentation was used as an apoptosis indicator. Results A23187 caused an increase in cytoplasmic calcium,loss of cell viability,and DNA fragmentation in PC12 cell line. Conclusion Increase in cytoplasmic calcium activates endonuclease(s) which causes DNA fragmentation at internucleosomal sites and neuronal apoptosis.

关 键 词：胞浆钙离子 细胞凋亡 核酸内切酶 神经细胞 

分 类 号：R339.3[医药卫生—人体生理学] R329.28[医药卫生—基础医学]