γ线与微波复合辐射对小鼠骨髓细胞增殖分化能力的影响  

Effect of γ-ray combined with microwave radiation on proliferation and differentiation potential of bone marrow cells in mice

作　　者：贾永峰[1,2] 王水明[1] 王德文[1] 彭瑞云[1] 高亚兵[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]内蒙古医学院附属医院病理科,呼和浩特010059

出　　处：《中国体视学与图像分析》2010年第2期165-170,共6页Chinese Journal of Stereology and Image Analysis

基　　金：国家自然科学基金重点项目(30230360)

摘　　要：目的探讨γ线复合微波辐射对小鼠骨髓细胞增殖和分化能力的影响及意义。方法 150只昆明小鼠随机分为对照组、微波辐射组、γ线辐射组、γ线复合微波辐射组,小鼠经5.5Gy60COγ线辐射后立即进行微波辐射(平均功率密度50mW/cm2、辐射时间5min),于辐射后6h、1d、3d、7d、14d,应用流式细胞术、Feulgen染色、免疫细胞化学结合图像分析技术检测骨髓细胞周期、DNA含量和PCNA表达,并进行粒-单系祖细胞集落(CFU-GM)和红系祖细胞集落(CFU-E)培养计数。结果与对照组比较,微波组于辐射后1dG2-M期细胞比例明显增高,6h～3dCFU-GM和CFU-E数明显减少;射线组和复合组于辐射后6h和1dS期细胞显著减少,G0-G1期和G2-M期细胞比例明显增高,1～7dPCNA表达明显降低;射线组于辐射后1d和3dDNA含量明显减少,复合组于辐射后6h～7d亦明显减少,并于1d明显少于射线组;射线组和复合组于辐射后14d内CFU-GM和CFU-E数显著减少,且复合组明显少于射线组。结论 5.5Gyγ线复合微波辐射通过影响细胞周期、下调PCNA表达、降低DNA合成,明显抑制骨髓细胞的增殖分化,本实验条件下,其损伤效应主要以γ射线为主,微波可加重其损伤。Objective To explore the effect of γ-ray combined with microwave radiation on proliferation and differentiation potential of bone marrow cells and its significance in mice. Methods 150 Kunming mice were divided into 4 groups of normal control, microwave radiation,γ-ray radiation,and γ-ray plus microwave radiation groups. Mice were irradiated by microwave at an average power density of 50 mW/cm2 for five minutes prior to exposure to 5.5 Gy 60CO γ-ray irradiation. Cell cycle was assessed by flow cytometry. DNA content and proliferating cell nuclear antigen （PCNA） expression of bone marrow cells were detected by Feulgen and immuocytochemical staining combined with image analysis. Colony forming unit-granulocyte and macrophage（CFU-GM） and colony forming unit-erythrocyte （CFU-E） were counted at 6 h, 1 d, 3 d, 7 d and 14 d after radiation. Results Compared with the nomal control gropup, there was a significant increase in G2-M cells at 1 d, and decrease in CFU-GM and CFU-E counts from 6 h to 3 d after microwave irradiation. cells S stages were significantly decreased, while cells in G0-G1 and G2-M stages were significantly increased at 6 h and 1 d, expression of PCNA was obviously lowered from 1 d to 7 d after irradiation in the γ-ray radiation group and γ-ray plus microwave radiation group. The DNA content was obviously decreased at 1 d and 3 d in the γ-ray radiation group, obviously decreased from 6 h to 7 d in γ-ray plus microwave radiation group, and was more obvious than that at 1 d after γ-ray radiation. There was a significant reduction in CFU-GM and CFU-E counts within 14 d in the γ-ray radiation group and more significant in the γ-ray plus microwave radiation group after irradiation. Conclusion Under the conditions used in this study, γ-ray plus microwave radiation may significantly inhibit the proliferation and differentiation potential of bone marrow cells in mice, through the effect on cell cycle, downregulation of PCNA expression and decrese of DNA synthesis. The changes are mainly

关 键 词：Γ射线 微波 骨髓细胞 细胞增殖 

分 类 号：R818[医药卫生—放射医学]