ApoC-Ⅲ启动子C-482T和T-455C单核苷酸多态性与2型糖尿病的相关性研究  

Study on relationship between single nucleotide polymorphism on C-482T and T-455C promoter of apoC-Ⅲ and type 2 diabetes mellitus

作　　者：袁瑾[1] 崔世维[1] 俞娟[2] 

机构地区：[1]南通大学附属医院内分泌科,江苏南通226001 [2]南通大学附属医院检验科,江苏南通226001

出　　处：《分子诊断与治疗杂志》2010年第5期332-336,共5页Journal of Molecular Diagnostics and Therapy

摘　　要：目的研究载脂蛋白CⅢ(apoC-Ⅲ)启动子单核苷酸多态性(SNP)C-482T和T-455C与中国江苏地区汉族人群2型糖尿病(T2DM)间的关系。方法测定中国江苏地区汉族人群164例T2DM患者及145例健康人群对照组apoC-Ⅲ的两个位点C-482T和T-455C的基因型和等位基因频率;同时测定两组人群的血脂谱。结果上述两个位点的基因型频率和等位基因在T2DM组和对照组间无统计学差异(P﹥0.05),两组间的apoA1和apoC-Ⅲ血清水平的差异有统计学意义(P=0.01和P<0.01),对照组中C-482T的TT基因的apoC-Ⅲ血清水平高于CC和CT基因型,差别有统计学意义(P<0.01)。结论 apoC-Ⅲ启动子的两个SNP位点C-482T和T-455C及单体型与2型糖尿病的发病无关,但两组间血脂谱有差别,健康人群的C-482T多态性与高apoC-Ⅲ血清水平相关。Objective To investigate the association between single nucleotide polymorphism （SNP） on C-482T and T-455C promoter of apoC-Ⅲ and type 2 diabetes mellitus（T2DM） in the Chinese of Han nationality in Jiangsu province. Methods 164 cases of T2DM and 145 healthy people of Han nationality in Jiangsu province were selected to detect the frequencies of genotypes and alleles of C-482T and T-455C of apoC--Ⅲ gene. The blood lipid profiles were assayed. Results There was no significant difference in frequencies of alleles or genotypes of apoC-Ⅲ between the two groups （P〉0.05）. There was significant difference in apoA1 and apoC-Ⅲ （P=-0.01 and P〈0.01）. -482TT has statistically significance on increased levels of apoC-Ⅲ compared to -482CC and -482CT（P〈0.01） in the group without T2DM. Conclusion The SNPs C-482T and T-455C of apoc-Ⅲ and the haplotypes are not associated with the incidence of T2DM. But there is the diversity of blood lipid profile. C-482T is associated with the increased levels of apo C-Ⅲ.

关 键 词：2型糖尿病 载脂蛋白CⅢ 单核苷酸多态性 

分 类 号：R587.1[医药卫生—内分泌]