从87种中草药植物中分离内生放线菌及其规律的初探  

Isolation of endophytic actinomycetes from 87 Chinese medicinal plants and summary of its possible rule

作　　者：田新莉[1] 钟莉[1] 成秋香[1] 陈振华[1] 周敏[1] 王韬[1] 范云[1] 杨勇[1] 郭鹏[1] 夏海洋[1] 覃重军[1] 

机构地区：[1]中国科学院上海生命科学研究院植物生理生态研究所合成生物学重点实验室,上海200032

出　　处：《中国抗生素杂志》2010年第9期659-662,706,共5页Chinese Journal of Antibiotics

基　　金：国家自然科学基金(30870067;30325003;30770045);863项目(2007AA021503);中科院项目(KSCX2-YW-G-014)资助

摘　　要：为了考察从植物中分离内生放线菌的规律,采集了87种中草药植物,包括62种草本植物和25种木本植物。减少系统误差后的统计数据显示,从55种草本植物和13种木本植物中各分离和初步鉴定了519株和41株内生放线菌,暗示从大多数草本植物比从木本植物中分离到内生放线菌的概率大。通过比较不同植物组织的出菌率,发现不论是草本还是木本植物,植物的地上部分(茎和叶)分离到的内生放线菌的数量比地下根部的多。这些初步的分离规律对于今后从中草药植物中高效地分离内生放线菌提供了借鉴意义。In order to investigate possible rule on isolation of endophytic actinomycetes, 87 Chinese medicinal plants including 62 herbal and 25 woody plants were collected. After reducing deviation, statistic data showed that 519 and 41 strains were isolated from 55 herbal and 13 woody plants respectively, indicating that the probability of isolating endophytic actinomycetes from herbal plants was more than that from woody plants. Comparing with different tissues, no matter from herbal or woody plants, endophytic actinomycetes strains were obtained more from stems/leaves than from roots. These preliminary summaries might help isolate efficiently endophytic actinomycetes from Chinese medicinal plants.

关 键 词：中草药植物 内生放线菌 

分 类 号：R939.132[医药卫生—生药学]