伴放线放线杆菌血液感染菌株的血清型分析  

PCR Serotyping of 29 Aggregatibacter actinomycetemcomitans Strains from Blood Isolates

作　　者：钟德钰[1] 章锦才[1] 张雄[1] Sirkka Asikainen 

机构地区：[1]广东省口腔医院.南方医科大学附属口腔医院牙周病科,广东广州510280 [2]瑞典于默奥大学牙学院

出　　处：《广东牙病防治》2010年第9期472-476,共5页Journal of Dental Prevention and Treatment

摘　　要：目的探讨不同血清型伴放线放线杆菌在全身血液感染中的分布规律。方法采用聚合酶链反应(polymerase chain reaction,PCR)方法对29株从全身感染患者血液中分离培养的伴放线放线杆菌菌株进行血清型分析,采用ATCC 29523(血清型a)、ATCC 43718(血清型b)、ATCC 33384(血清型c)、IDH781(血清型d)、IDH1705(血清型e)以及CU1000(血清型f)作为伴放线放线杆菌参考菌株。根据伴放线放线杆菌不同血清型特异性多糖抗原基因序列设计6对不同的寡核苷酸引物,用这6对引物分别对上述菌株的DNA进行PCR扩增分析。结果每一对引物均针对相应血清型的参考菌株产生特异性单一条带PCR产物,血清型a、b、c、d、e、f菌株的PCR产物大小分别为428、298、559、690、211、232bp。29株血源性临床分离菌株中血清型b16株(55%),血清型a和c各4株(14%),血清型d和f各2株(7%),还有1株无法鉴定为上述任何一种血清型。结论血液中分离培养的伴放线放线杆菌以血清型b为主,其次是血清型a和c。Objective To determine serotypes of 29 Aggregatibacter actinomycetemcomitans strains from blood isolates by polymerase chain reaction. Methods ATCC 29523 （serotype a）, ATCC 43718 （serotype b）, ATCC 33384 （serotype c）, IDH 781 （serotype d）, IDH 1705 （serotype e） and CU 1000 （serotype f） were used as reference strains. Six pairs of oligonucleotide primers specific for gene clusters involved in the biosynthesis of serotype specific polysaccharide antigens were designed. The specificity of the primers were evaluated by the reference strains of Aggregatibacter actinomycetemcomitans. Serotypes of 29 Aggregatibacter actinomycetemcomitans strains from blood iaolates were determined by PCR with the primers. Results Each pair of primers can specifically identify one serotype of Aggregatibacter actinomycetemcomitans. No cross reaction was observed in all strains. The PCR product sizes were： 428 bp （serotype a）, 298 bp （serotype b）, 559 bp （serotype c）, 690 bp （serotype d）, 211 bp （serotype e） and 232 bp （serotype f）. Of the 29 strains from blood isolates, 16 strains were serotype b （55%）, 4 strains were serotype a （14%）, 4 strains were serotype c （14%）, 2 strains were serotype d （7%）, 2 strains were serotype f （7%）. One strain could not be serotyped by PCR assay. Conclusion Serotype b was the most frequently detected serotype of the Aggregatibacter actinomycetemcomitans strains from blood isolates. Serotype a and c could be detected more often than the other serotypes.

关 键 词：伴放线放线杆菌 血液感染 血清型 HACEK 

分 类 号：Q939.1[生物学—微生物学]