生物大分子在纳米微孔中迁移运动的研究进展  

Progress in study on translocation of biomacromolecules through a nanopore

作　　者：冯剑[1] 尚亚卓[2] 周丽绘[2] 刘洪来[2] 

机构地区：[1]滁州学院化学与生命科学系,安徽滁州239012 [2]化学工程联合国家重点实验室,华东理工大学化学系,上海200237

出　　处：《化工学报》2010年第10期2501-2509,共9页CIESC Journal

基　　金：国家自然科学基金项目(20736002,20706013);化学工程联合国家重点实验室开放课题项目(SKL-ChE-09C02)~~

摘　　要：DNA等带电生物大分子通过各种生物膜上的纳米微孔的迁移是生命现象中的关键运动步骤之一,对这种迁移过程的研究不仅有助于了解生命运动的分子机理,在快速DNA测序、基因治疗和药物传输的控制等方面也有潜在应用价值。本文介绍了近年来关于DNA分子通过纳米微孔迁移过程在实验测定、理论分析和计算机模拟等方面的研究进展,重点关注迁移动力学及其机理,特别是迁移时间和分子链长间的标度关系等。Translocation of charged biological macromolecules such as DNA across biological membranes through a nanopore is one of the most crucial elementary processes of life.Study on the translocation of DNA is helpful to understand the molecular mechanism of life and explore its potential application in rapid DNA sequencing,gene therapy,drug delivery and so on.In this paper,the progresses in the investigation of translocation process of DNA through nanopore by using single-molecule electrophysiology experiments,theory of polymer transport and molecular simulations are reviewed.The translocation dynamics of DNA and its mechanism are paid more attention to,especially,the scaling relationship of translocation time with molecular weight.

关 键 词：DNA 蛋白质 迁移 纳米微孔 分子模拟 

分 类 号：TQ028.7[化学工程]