番茄幼苗黄化曲叶病毒带毒率检测  

Detection of Toxicity Rate of Tomato yellow leaf curl virus (TYLCV) Geminiviruses in Tomato Seedlings

作　　者：师迎春 李子龙[2] 王艳辉 李荣华[4] 许小荣[5] 崔文清 陈善义[2] 张瑞[2] 姚丹丹 郑建秋 周涛[2] 

机构地区：[1]北京市植物保护站,北京100029 [2]中国农业大学植物病理学系,农业部植物病理学重点实验室,北京100193 [3]北京市平谷区植物保护站,北京101300 [4]北京市顺义区植保植检站,北京102600 [5]北京市大兴区植保植检站,北京101200 [6]北京市通州区植物保护站,北京101117

出　　处：《中国蔬菜》2010年第18期76-79,共4页China Vegetables

基　　金：北京市果类蔬菜产业创新团队病虫防控研究室资助

摘　　要：2009年番茄黄化曲叶病毒病在北京市首次发生,且给生产造成严重损失,为了解番茄苗期带毒率以便进行综合防控,2010年2～3月在顺义区、大兴区、通州区、平谷区番茄育苗温室采集即将定植及已定植1～2d的番茄幼苗植株进行黄化曲叶病毒分子检测。共检测番茄幼苗样品645株,检出阳性样品226个,番茄幼苗带毒率为35.04%。检测结果表明,被侵染的番茄幼苗不表现明显症状,病毒含量明显低于表现典型症状的成株番茄。根据检测结果,初步预测2010年北京市早春温室番茄黄化曲叶病毒病有可能较大程度发生。Tomato yellow leaf curl virus（TYLCV）was first occurred in Beijing in 2009 and induced severe damage to tomato production.To predict and control this disease in Beijing,detection of whitefly transmitted geminiviruses in tomato seedlings was carried out.During February to March of 2010,tomato seedlings were collected in greenhouses from counties of Shunyi,Daxing,Tongzhou and Pinggu in Beijing.Altogether 645 seedling samples were collected and 226 seedling samples were positive in detection,which showed that 35.04 % of the seedlings were infected by whitefly transmitted geminiviruses.At the same time,the results indicated that there were no typical leaf curl symptoms on infected tomato seedlings and lower virus accumulation than in adult tomato plants with typical symptom.Based on this detection,we preliminarily forecast that this disease may occur with larger scale in early spring greenhouse tomato cultivation in 2010.

关 键 词：番茄黄化曲叶病毒病 幼苗 带毒率 

分 类 号：S641.2[农业科学—蔬菜学]