表达鸡马立克氏病病毒糖蛋白B基因重组鸡痘病毒毒种的纯净性检验及冻干疫苗的保存期测定  

Purity Assay of the Original Recombinant Fowlpox Virus Expressing gB Gene of Marek's Disease Virus and Determination of Valid Storage Time of Lyophilized rFPV-gB/R Vaccine

作　　者：卢军[1,2] 胡传伟[2] 金乔[2] 刘武杰[2] 彭大新[2] 刘秀梵[2] 

机构地区：[1]上海市动物疫病预防控制中心,上海201103 [2]农业部畜禽传染病学重点开放实验室,江苏扬州225009

出　　处：《中国兽药杂志》2010年第9期1-3,共3页Chinese Journal of Veterinary Drug

基　　金：农业科技成果转化基金项目(02EFN213201078)

摘　　要：为了检测高效表达鸡马立克氏病病毒gB基因的重组鸡痘病毒(rFPV-gB/R)的纯净性,对纯化后的0代重组病毒毒种进行了细菌和支原体检验;并对中间试制的冻干疫苗在-20℃保存期进行了测定。结果表明,重组病毒毒种高度纯净,没有污染任何细菌或支原体,符合新制品制造和检验规程申报的要求;中试冻干疫苗在-20℃保存了12个月,病毒含量始终大于109PFU/mL,疫苗保存期可达12个月。Purity assay was performed with the original recombinant fowlpox virus expressing gB gene of Marek＇s disease virus,and the valid storage time of pilot product of lyophilized rFPV-gB/R vaccine was determined.The results showed that rFPV-gB/R was free from exogenous bacterial and mycoplasma,which was in accordance with the requirements of new product manufacture and regulation declaration.The experiment of determining the valid storage time of the vaccine demonstrated that the vaccine product remained effective for 12 months at preservation temperature of-20℃.

关 键 词：鸡马立克氏病 GB基因 重组鸡痘病毒 纯净性检验 保存期 

分 类 号：S852.65[农业科学—基础兽医学]