Quantitative Analysis of Components in OC-CS Sprays by High Performance Liquid Chromatography with Double Wavelength UV Detection  

作　　者：孙立权 陈振贺 邱日祥 王莉晶 钱伟 耿利娜 罗爱芹 

机构地区：[1]School of Life Science,Beijing Institute of Technology [2]The First Research Institute of Ministry of Public Security

出　　处：《Defence Technology（防务技术）》2010年第3期216-219,共4页Defence Technology

基　　金：Sponsored by the Basic Research Funds of Beijing Institute of Technology (20081642007)

摘　　要：The method of reversed-phase high performance liquid chromatography was established for the determination of effective components in OC-CS spray using double wavelength UV detection.The method was utilized under following conditions:a column of Kromasil C18(250 mm × 4.6 mm,5 μm),mobile phase consisting of methanol/water(80 /20) at a flow rate of 0.8 mL/min,column temperature of 25 ℃,and the UV detection at 227 nm and 300 nm.Three key components in OC-CS spray could be distinguished clearly,including o-chlorobenzalmalononitrile(CS),oleoresin capsicum(OC) and dihydrocapsaicin(DC).This method has the advantages of fast,simple and satisfactory linear relationship between UV absorption and concentration.It may be considered to turn into a standard method for detection of related components in the spray.The method of reversed-phase high performance liquid chromatography was established for the determination of effective components in OC-CS spray using double wavelength UV detection. The method was utilized under following conditions： a column of Kromasil C18 （250 mm× 4.6 mm, 5μm） , mobile phase consisting of methanol/water （80/20） at a flow rate of 0. 8 mL/min, column temperature of 25℃ , and the UV detection at 227 nm and 300 nm. Three key components in OC-CS spray could be distinguished elearly, including o-ehlorobenzalmalononitrile （CS）, oleoresin capsicum （OC） and dihydrocapsaicin （ DC ）. This method has the advantages of fast, simple and satisfactory linear relationship between UV absorption and concentration. It may be considered to turn into a standard method for detection of related components in the spray.

关 键 词：analytical chemistry OC-CS sprays o-chlorobenzalmalononitrile oleoresin capsicum dihydrocapsaicin high performance liquid chromatography 

分 类 号：O657.72[理学—分析化学]