大鼠大脑海马注射β-淀粉样蛋白对caspase-3蛋白表达的影响及通络救脑口服液的干预作用  

Expression of protein caspase-3 in Aβ1-40 induced AD rats and effect of TongluoJiu'nao Oral Solution

作　　者：张晓杰[1] 董海影[1] 孙玉荣[1] 李澎涛[2] 

机构地区：[1]齐齐哈尔医学院病理教研室,齐齐哈尔101006 [2]北京中医药大学基础医学院病理教研室,北京100029

出　　处：《中华中医药杂志》2010年第10期1678-1680,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：齐齐哈尔市科学技术计划项目(No.SF-0805)~~

摘　　要：目的:探讨大鼠大脑海马立体定向注射β-淀粉样蛋白1-40(Aβ1-40)对caspase-3蛋白表达的影响及通络救脑口服液的干预作用。方法:140只SD大鼠随机分为空白对照组、假手术组、模型组、盐酸多奈哌齐组、通络救脑口服液低、中、高剂量组(12、24、48mg.kg-1.d-1)共7组。采用大脑海马立体定向注射凝聚态Aβ1-40诱导老年性痴呆动物模型,药物干预4周后,电镜观察海马组织超微结构的变化,实时免疫印迹法检测caspase-3蛋白的表达。结果:电镜观察结果显示:模型组大鼠海马神经元变性,通络救脑口服液能改善大鼠海马神经元病理改变。免疫印迹结果显示:与假手术组比较,模型组大鼠海马组织caspase-3蛋白表达显著升高(P<0.05);与模型组比较,通络救脑口服液组(12、24、48mg.kg-1.d-1)大鼠海马组织caspase-3蛋白表达均显著减少(P<0.05)。结论:海马注射Aβ1-40后大鼠海马组织caspase-3蛋白表达水平明显增高,而通络救脑口服液可以抑制海马组织促凋亡基因caspase-3蛋白的表达,从而发挥其抗老年性痴呆神经细胞凋亡的作用。Objective：To observe the effect of TLJN Oral Solution on protein caspase-3 expression in brain tissue of amyloid-β1-40 injection into brain induced experimental AD rats in vivo.Methods：140 SD rats were enrolled in the study,and they were equally randomly divided into blank controls group,sham controls group,negtive control group,positive control group and experiment group（12mg？kg-1？d-1,24mg？kg-1？d-1,48mg？kg-1？d-1）,A rat model of AD induced by amyloid-β1-40 sterotaxis injection the bilateral Hippocampus.All rats were sacrificed at 5 week after treatment for 4 weeks.Change of hippocampus is observed by electron microscope.Protein caspase-3 expression in brain tissue was assayed by immunoblotting test.Results：Hippocampus is found denatured in model rats,and TLJN Oral Solution can alleviate the damage of hippocampus neurons.The protein caspase-3 expression was up-regulate expression in model groups compared with the sham group（P0.05）.In experiment groups the protein caspase-3 expression was down-regulate expression compared with the model group（P0.05）.Conclusions：amyloid-β1-40 injection the bilateral Hippocampus can lead to the over-regulate expression of protein caspase-3 in hippocampus tissue.TLJN Oral Solution may down-regulate expression of protein caspase-3.

关 键 词：阿尔茨海默氏病 通络救脑口服液 caspase-3 

分 类 号：R285.5[医药卫生—中药学]