辣椒冷诱导差异表达基因分析  

Cloning of Genes Related with Cold Tolerance from Capsicum annuum L.

作　　者：朱文超[1] 巩振辉[1] 陈儒钢[1] 李大伟[1] 尹延旭[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨陵712100

出　　处：《西北植物学报》2010年第9期1733-1737,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金："十一五"国家科技支撑计划项目(2008BADB1B04);陕西省"13115"科技创新工程重大科技专项(2008ZDKG-03);中国博士后科学基金(20090451404);中国博士点基金(20090204120005);西北农林科技大学"青年学术骨干支持计划"(01140304);陕西省蔬菜产业技术体系项目(sg-3)

摘　　要：采用cDNA-AFLP技术分析了4℃低温诱导前后耐寒辣椒(Capsicumannuum L.)品系P70的基因表达差异谱。结果获得了120个差异片段,对阳性克隆中的12个差异片段进行测序和Blast-x比对,发现其中有4个片段(KH-1、KH-2、KH-3和KH-4)与抗逆性相关,其碱基数分别为185、160、269和511 bp,4个片段分别与编码抗坏血酸过氧化物酶基因的同源性达98%、与细胞色素p450基因的同源性达98%、与牛血清蛋白(BSA)基因的同源性达94%、与水孔通道蛋白(AQP)基因的同源性达90%。4个差异基因表达模式为:基因KH-1、KH-2、KH-3上调表达,KH-4下调表达。P70（Capsicum annuum L.） is a pepper line with excellent chilling-tolerance.The gene expressions of P70 stressed at 4 ℃ for different times were studied via cDNA-AFLP.One hundred and twenty differential expression cDNA fragments were obtained,twelve positive fragments of which were sequenced and analyzed by Blast-x.The blast showed that four sequenced fragments（KH-1 about 185 bp,KH-2 about 160 bp,KH-3 about 269 bp and KH-4 about 511 bp） were similar to the known genes of chilling-tolerance in the database,in which KH-1,KH-2,KH-3 and KH-4 had high homology to ascorbic acid peroxidase gene（98%）,cytochrome p450 gene（98%）,bovine serum albumin（BSA）gene（94%）and water-hole channel protein（AQP） gene（90%）,respectively.The differential expression of cDNA also showed that KH-1,KH-2,KH-3 were up-regulated and KH-4 was down-regulated under the cold stress.These genes will reveal the mechanism for cold tolerance.

关 键 词：辣椒 CDNA-AFLP 低温诱导 

分 类 号：Q786[生物学—分子生物学]