Deg1 is involved in the degradation of the PsbO oxygen-evolving protein of photosystem Ⅱ in Arabidopsis  

作　　者：LI Jin SUN XuWu ZHANG LiXin 

机构地区：[1]School of Life Sciences, Lanzhou University, Lanzhou 730000, China [2]Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

出　　处：《Chinese Science Bulletin》2010年第27期3145-3148,共4页

基　　金：supported by the State Key Basic Research Program of China(2006CB910300);the National Natural Science Foundation of China(30725003)

摘　　要：Deg1,a thylakoid lumen-localized protease,retains both chaperone and protease activities.The in vivo function of Deg1 has been shown to be involved not only in PSII assembly but also in the degradation of PSII reaction center protein D1.Here we used the transgenic plants with reduced Deg1 to examine whether the lumen-localized proteins are also the substrates of Deg1 in vivo.Our results showed that the transgenic plants accumulated degradation products of the PsbO protein while the levels of full-length PsbO were not affected.The PsbO degradation products could be efficiently degraded by the recombinant Deg1.These results suggest that Deg1 is involved in the degradation of the PsbO degradation fragments,but not in the initial cleavage event itself.Degl, a thylakoid lumen-localized protease, retains both chaperone and protease activities. The in vivo function of Degl has been shown to be involved not only in PSII assembly but also in the degradation of PSII reaction center protein D1. Here we used the transgenic plants with reduced Degl to examine whether the lumen-localized proteins are also the substrates of Degl in vivo. Our results showed that the transgenic plants accumulated degradation products of the PsbO protein while the levels of full-length PsbO were not affected. The PsbO degradation products could be efficiently degraded by the recombinant Degl. These results suggest that Degl is involved in the degradation of the PsbO degradation fragments, but not in the initial cleavage event itself.

关 键 词：降解产物 蛋白酶 光系统II 拟南芥 放氧 转基因植物 转基因植株 类囊体腔 

分 类 号：Q945.11[生物学—植物学] TJ92[兵器科学与技术—武器系统与运用工程]