聚球藻7942混养培养中碳代谢与能量利用  

Carbon metabolism and energetic utilization of Synechococcus sp.PCC7942 under mixotrophic condition

作　　者：颜日明[1,2] 张志斌[2] 曾庆桂[2] 朱笃[2,3] 储炬[1] 

机构地区：[1]华东理工大学生物工程学院生物反应器工程国家重点实验室,上海200237 [2]江西师范大学生命科学学院江西省亚热带植物保护与利用重点实验室,南昌330022 [3]宜春学院,宜春336000

出　　处：《生物工程学报》2010年第9期1239-1248,共10页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.20506009);江西省自然科学基金(No.0530095);江西省教育厅(No.GJJ08147)资助~~

摘　　要：为了考察聚球藻7942在混养条件下的能量利用效率,分别以葡萄糖和乙酸为碳源开展了聚球藻7942的混养培养研究,并在此基础上利用代谢通量分析方法对聚球藻7942混养条件下的碳代谢和能量利用进行了探讨。结果表明:葡萄糖和乙酸均能促进藻细胞生长,且乙酸促进藻细胞生长的作用更为明显;葡萄糖利用可明显增加藻细胞糖酵解途径中碳代谢流量,而乙酸利用则导致糖酵解途径中碳代谢流量减小,两种有机碳源均增加了柠檬酸循环中碳代谢流量;有机碳源导致藻细胞光化学效率下降,而葡萄糖较之乙酸降低藻细胞光化学效率更为明显。虽然混养条件下光能的贡献率要小于光自养,但基于能量的细胞得率和能量转换率均高于光自养,光自养和以葡萄糖、乙酸为碳源的混养中基于ATP生成的能量转换效率分别为6.81%、7.43%和8.77%。To investigate the energy utilization efficiency of Synechococcus sp. PCC7942 under mixotrophic conditions,we studied its growth characteristics in mixotrophic cultures with glucose and acetic acid respectively and discussed the carbon metabolism and energy utilization based on metabolic flux analysis. Results showed that both glucose and acetate could better enhance the growth of Synechococcus sp. PCC7942,and the latter was more effective. The metabolic flux through glycolytic pathway in mixotrophic cultures was stimulated by glucose whereas depressed by acetate,while the flux through the tricarboxylic acid cycle increased in both cases. Under mixotrophic conditions,glucose makes more significant impact on the diminishment of photochemical efficiency of Synechococcus sp. PCC7942. Although the contribution of light energy was smaller,the cell yields based on total energy in mixotrophic cultures were higher comparing with photoautotrophic culture. The energy conversion efficiencies based on ATP synthesis in photoautotrophic culture,mixotrophic cultures with glucose and with acetate were evaluated to be 6.81%,7.43% and 8.77%,respectively.

关 键 词：聚球藻7942 混养培养 代谢通量分析 碳代谢 能量利用 

分 类 号：Q943.1[生物学—植物学]