结核性胸液T细胞组成和细胞因子表达的探讨  

Phenotypic and functional characteristics of T cells in tuberculosis pleurisy

作　　者：劳穗华[1] 李丽[2] 李琴[2] 张贤兰[1] 乔丹[2] 付笑迎[2] 吴长有[2] 

机构地区：[1]广州市胸科医院,广州510095 [2]中山大学中山医学院免疫学教研室热带病防治研究教育部重点实验室,广州510080

出　　处：《中国免疫学杂志》2010年第9期839-841,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(30872300)

摘　　要：目的:探讨结核性胸液中CD3+ T、CD3+ CD4+ T、CD3+ CD8+ T细胞和CD4+/CD8+比值及细胞因子表达。方法:分离外周血单个核细胞和胸液细胞,流式细胞仪检测CD3+、CD3+ CD4+、CD3+ CD8+和CD4/CD8比值。ELISA法检测胸液上清中IFN-γ、IL-2和TNF-α的水平。流式细胞仪检测CD3+ CD4+和CD3+ CD8+ T细胞Th1细胞因子IL-2、TNF-α、IFN-γ的产生。结果:与外周血相比,胸液细胞CD3+ CD4+ T和CD3+ T细胞比例显著增加。ELISA结果表明,胸液上清中存在大量Th1型细胞因子。流式细胞仪分析结果表明,IL-2、TNF-α和IFN-γ主要由CD3+ CD4+ T细胞产生,而CD3+CD8+T细胞较少或不分泌细胞因子。结论:结核病人胸液中CD3+和CD3+ CD4+ T细胞比例增加,胸液中存在大量Th1细胞因子,且主要来源于CD3+ CD4+ T细胞亚群,可能在抗结核免疫中发挥重要作用。Objective：To elucidate the phenotypic and functional characteristics of CD3＋ CD4＋ and CD3＋ CD8＋ T in pleural fluid of patients with tuberculous pleurisy.Methods：PBMCs and PFCs were isolated. The frequency of CD3＋,CD3＋ CD4＋,CD3＋ CD8＋ T cells and the ratio of CD4/CD8 in PFCs were analyzed by FACS. The concentration of IFN-γ,IL-2 and TNF-α in the pleural fluid were analyzed by ELISA.Production of IFN-γ,IL-2 and TNF-α by CD3＋ CD4＋ and CD3＋ CD8＋ T cells was detected by FACS following stimulation with BCG.Results：Higher frequencies of CD3＋ CD4＋ T cells and CD4＋ T/CD8＋ T ratio were demonstrated in PFCs when compared with PBMCs from normal donors and TB patients.ELISA analysis demonstrated that significantly high levels of IFN-γ and TNF-α were detected in pleural fluid.Further analysis by FACS indicated that IL-2,TNF-α and IFN-γ were predominantly secreted by CD3＋CD4＋T cells but not by CD3＋ CD8＋ T cells.Conclusion：The frequencies of CD3＋ and CD3＋ CD4＋ T cells are increased in PFCs. Large amounts of Th1 cytokines are detected,which are produced by CD3＋ CD4＋ T cells,and might play important roles against TB infection.

关 键 词：结核性胸膜炎 细胞因子 T细胞 

分 类 号：R392[医药卫生—免疫学]