鸡IFN-γ与鸡柔嫩艾美耳球虫3-1E抗原共表达DNA疫苗的免疫保护性研究  

Protective effect of a DNA vaccine co-expressing both chicken IFN-γ and E.tenella 3-1E

作　　者：徐守振[1] 潘保良[2] 

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109 [2]中国农业大学动物医学院,北京海淀100193

出　　处：《中国兽医杂志》2010年第9期5-7,共3页Chinese Journal of Veterinary Medicine

基　　金：青岛农业大学高层次人才启动基金(630739)

摘　　要：采用SOE-PCR将鸡柔嫩艾美耳球虫3-1E抗原基因与鸡IFN-γ基因用(GGGGS)3接头融合,扩增出IFN-γ/3-1E融合基因,将其克隆到真核表达载体proVAX中构建双价融合表达质粒proIE。通过IFA检测proI、proE、proIE重组质粒在转染细胞PK-15后的蛋白表达情况。将重组质粒于14、21日龄免疫AA肉鸡,28日龄感染5×104个柔嫩艾美耳球虫孢子化卵囊,观察免疫保护效果。结果显示,与proE和proI相比,proIE双价融合DNA疫苗具有增强免疫保护作用,可显著降低粪便中的卵囊排出量(P<0.05),但体重增加并不显著(P>0.05)。The IFN-γ/3-1E fusion gene encoding E.tenella 3-1E antigen and chicken IFN-γ linked with Gly4Ser 3 was amplified by SOE-PCR,and the fusion gene was subcloned into the mammalian expression vector proVAX forming the chimeric plasmid proIE.The expressed proteins of purified plasmids transfected PK-15 cells were detected by IFA.Broilers were vaccinated with the DNA vaccines respectively at the age of 14 and 21 days.Chickens were challenged with 5×104 E.tenella sporulated oocysts at 28 days and the immune protective efficacy was observed.The results indicated that the proIE showed the enchanced protective immunity as demonstrated by significantly reduced oocyst shedding compared with proE and proI（P0.05）,but the proIE did not have an effect of body weight gain（P0.05）.

关 键 词：柔嫩艾美耳球虫 3-1E基因 IFN-Γ基因 DNA疫苗 免疫保护 

分 类 号：S852.723[农业科学—基础兽医学]