数字共焦显微仪序列光学切片自动采集方法研究  

Research on the method of auto-collecting serial optical sections for digital confocal microscope

作　　者：聂雄[1] 陈华[1] 

机构地区：[1]广西大学计算机与电子信息学院,南宁530004

出　　处：《仪器仪表学报》2010年第9期2148-2153,共6页Chinese Journal of Scientific Instrument

基　　金：广西高校人才小高地建设创新团队资助计划(桂教人[2007]71号);广西科学研究与技术开发计划项目(桂科攻0992002-23);南宁市科学研究与技术开发计划(200801028A);广西大学科学基金(X071005)资助项目

摘　　要：提出一种基于图像处理的数字共焦显微仪序列光学切片自动采集方法。方法分两个步骤:第一步是对细胞进行自动聚焦;第二步是细胞的起始点自动调焦定位及进行光学切片。方法采用清晰度评价函数作为判别标准,将灰度差分算法与拉普拉斯函数法相结合,通过计算机实现两个步骤的自适应调焦控制,进而实现光学切片的自动采集。实验结果表明,方法有较高的自动聚焦和细胞起始点定位的速度和准确性,对三维球形细胞序列光学切片图像采集,简单易行,效果良好。A method of auto-collecting serial optical sections based on image processing for digital confocal microscope is proposed.The method has two steps： the first step is auto-focusing to the cell,and the second step is focusing automatically to the starting point and terminal point of the cell and automatic optical sectioning.In this method,the definition criterion function is regarded as an estimation criterion of focusing,and gray scale difference method and Laplacian function method are combined to control the adaptive focusing in the two steps,and to achieve auto-collecting serial optical sections.Experiment results show that faster speed and higher accuracy in auto-focusing and locating start and terminal points are reached;and the proposed method is simple,easy and has good effect in collecting serial optical sections for three dimensional spherical cells.

关 键 词：数字共焦显微仪 光学切片采集 清晰度评价函数 自动聚焦 起终点定位 

分 类 号：TP391[自动化与计算机技术—计算机应用技术]