用PCR方法诊断鸡传染性鼻炎的研究 Ⅱ.病料保存及治疗对诊断的影响  

Further Studies on the PCR Test for Diagnosis of InfectiousCoryza II. Specimen Storage and Antibiotic Treatment

作　　者：陈小玲[1] 宋程[1] 龚玉梅[1] 

机构地区：[1]北京市农林科学院畜牧兽医研究所,北京100081

出　　处：《中国兽药杂志》1999年第2期6-8,共3页Chinese Journal of Veterinary Drug

摘　　要：围绕副鸡嗜血杆菌（Ｈｐｇ）分离和ＰＣＲ检验中遇到的病料运输保存及药物治疗影响问题进行试验设计， 结果证明将棉拭子放在甘油ＰＢＳ中４℃或直接取病鸡头冷藏运输保存对Ｈｐｇ 分离和ＰＣＲ 检验是最为简便有效的方法，Ｈｐｇ 可存活３～７ｄ，ＰＣＲ 检验到９０或３０ｄ 仍很敏感。试验还表明，抗菌药物治疗不仅影响细菌分离，也影响ＰＣＲ 检测。The experiments were designed to search both the suitable storage condition of the specimen collected from the infectious coryza (IC) diseased fourls in field cases and the influence of antibiotics treatment of the diseased on the pathogen culturing and PCR detection for disease diagnosis. The results showed that swabs stored in glycerol PBS as well as the chicken head stored in 4℃ were the optimum methods. With theses methods the pathogen Haemophilus paragallinarum survived for three days and the PCR test may be used for diagnosis even when specimen having been stored for 30 or 90 days. Antibitic treatment affected not only the pathogen isolation but also the PCR test.

关 键 词：鸡 传染性鼻炎 PCR 诊断 病料保存 治疗 

分 类 号：S858.317.7[农业科学—临床兽医学]