高粱非编码区SSR引物设计以及e-PCR的验证  

Design of SSR Primers and Verification of E-PCR in Non-coding Regions of Sorghum Genome

作　　者：陆景标[1,2] 李杰勤[1] 卢杰[1,2] 詹秋文[1] 

机构地区：[1]安徽科技学院,安徽凤阳233100 [2]安徽农业大学,合肥230036

出　　处：《种子》2010年第9期1-6,12,共7页Seed

基　　金：国家自然科学基金(No:31071470);科技部农业科技成果转化资金项目(No:2008GB2C300125);安徽省"115"产业创新团队资助(No:皖人才办[2009]2号);国家科技支撑计划项目(No:2008BADB3B10);安徽省高校学科拔尖人才基金(No:教秘人[2005]79号);安徽科技学院稳定人才专项(No:ZRC2009236)

摘　　要：本研究通过在NCBI公共数据库的高粱全基因序列中进行简单重复序列SSR搜索,结果显示共有87950个SSR位点,共有1134种重复,其中二元碱基和三元碱基重复所占比例最大,分别占43.36%,32.38%。根据SSR搜索结果设计了高粱SSR引物,通过两次e-PCR验证和筛选引物,随机合成了50对引物。以先锋和乐食两个高丹草品种为材料进行引物的筛选和评价。结果表明,50对引物中有46对引物出带,但只有8对引物具有多态性。这说明在高粱非编码区设计的引物多态性并不高于EST-SSR引物,但对高粱的SSR引物的开发可以起到补充和促进作用。The complete genome sequence of sorghum which downloaded from the NCBI public databases was used for a simple sequence repeated SSR search. The result showed that there were 87 950 SSR loci and 1 134 kinds of repetition about the results in all. The proportion of dinucleotide and trinucleotide repeats was placed on the first place, accounting for 43.36% and 32.38% of the all. SSR primers of sorghum were designed ba- sing on the search results of SSR, according to use the e-PCR to validate and screen primers two times ,50 pairs of primers were arbitrarily selected and synthesized to evaluate these primers by sorghum-sudangrass hybrids such as Xian-Feng and Le-Shi. The results showed that there were 46 target bands among 50 pair primer, and the target bands were characterized clearly and steadily, of which only 8 pairs had polymorphism. It indicated that the polymorphism of sorghum designed primers in non-coding region was not higher than the EST-SSR primers, but it played a complementary and catalytic role on the development of SSR primers in sorghum.

关 键 词：高粱 非编码区 SSR e—PCR 多态性 

分 类 号：S514[农业科学—作物学]