双阳离子咔唑衍生物与DNA相互作用的光谱研究  

Interaction Between Dicationic Carbazole Derivatives and Nucleic Acid by Spectrum

作　　者：张元红[1] 孙渝明[2] 刘鑫[1] 赵宁[1] 于晓强[1] 黄柏标[1] 

机构地区：[1]山东大学晶体材料国家重点实验室 [2]山东大学光学系,济南250100

出　　处：《高等学校化学学报》2010年第9期1860-1863,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:50673053;NSFC/RGC50218001;50173015;20973102)资助

摘　　要：合成了一种新型的双阳离子咔唑衍生物3,6-双(1-羟乙基-4-烯基吡啶)咔唑碘盐(3,6-BHVC),并对其进行了表征.利用紫外-可见吸收光谱、圆二色谱、单光子和双光子荧光发射光谱探讨了该化合物与DNA的结合方式.所有光谱测试结果表明,该化合物在[磷酸根]/[染料]比值较低的条件下主要以插入方式与DNA结合,在该比值较高的条件下主要以沟槽结合方式为主.表明咔唑衍生物可以成为一种探索具有高结合能力双光子DNA探针的基本结构.In this paper,3,6-bis （ 1-hydroxyethyl-4-vinylpyridinium） carbazole diiodide （ 3,6-BHVC） ,a novel dicationic carbazole derivative,was synthesized and characte-rized. Using electronic absorption spectra, circle dichroism spectra and one-and two-photon emission spectra,the binding modes between 3,6-BHVC and DNA were studied. All spectral results show that 3,6-BHVC intercalates into DNA base pairs at lower ratio of [phosphate]/[3,6-BHVC]and binds in DNA groove at higher ratio. This indicates that carbazole derivative can act as a primary structure to explore two-photon DNA probes with high binding affinity.

关 键 词：双阳离子咔唑衍生物 双光子细胞核荧光探针 结合方式 

分 类 号：O631.23[理学—高分子化学] O631.5[理学—化学]