聚乙二醇干扰素α-2a对乙型肝炎病毒e抗原阳性慢性乙型肝炎患者记忆性CD8^＋T淋巴细胞CD127表达影响的动态研究  

Dynamic analysis of CD127 expression on the memory CD8^＋ lymphocytes of hepatitis Be antigen positive chronic hepatitis B patients treated with peginterferon α-2a

作　　者：应灵军[1] 郑临[1] 吕国才[1] 徐陈槐[1] 吴炜[1] 杨益大[1] 

机构地区：[1]浙江大学医学院附属第一医院传染病科,杭州310003

出　　处：《中华传染病杂志》2010年第9期541-545,共5页Chinese Journal of Infectious Diseases

基　　金：国家“十一五”重大专项资助项目（2008ZX10002005-ZY004）;浙江科技厅面上社会发展项目（2009C33009）;浙江省中医药管理局一般资助项目（2008CA044）

摘　　要：目的 观察聚乙二醇干扰索α-2a抗病毒治疗对HBeAg阳性慢性乙型肝炎患者记忆性CD8^＋T淋巴细胞CD127分子表达的影响.方法 30例HBeAg阳性慢性乙型肝炎患者接受聚乙二醇干扰素α-2a治疗,每周1次,共48周.根据CD45RA、CD27分子表达来判定记忆性CD8^＋T淋巴细胞,以流式细胞术检测CD127在CD8^＋T淋巴细胞上的表达.组间均数比较采用Mann-Whitney检验.结果 HBeAg阳性慢性乙型肝炎患者在CD45RA-CD27＋记忆性CD8^＋T淋巴细胞上CD127的表达较健康对照组明显降低（Z=2.889,P＜0.05）,其表达水平与血清HBV DNA水平和HBeAg呈负相关.聚乙二醇干扰素α-2a抗病毒治疗应答较佳者随着HBV DNA和HBeAg水平的下降,记忆性CD8^＋T淋巴细胞表面CD127分子的表达在治疗24、48和72周都明显增加,而应答欠佳者则无明显变化（Z24周=1.954,Z48周=2.789,Z72周=2.989;均P＜0.05）.结论 慢性乙型肝炎患者通过有效的抗病毒治疗,CD8^＋T淋巴细胞表达CD127增加,CD127在记忆性CD8^＋T淋巴细胞上的表达水平可作为判定抗病毒治疗有效的重要标志.Objective To analyze CD127 expression on the memory CD8^＋ lymphocytes from hepatitis B e antigen （HBeAg） positive chronic hepatitis B （CHB） patients treated with peginterferon α-2a （Pegasys）. Methods Thirty HBeAg positive CHB patients were treated with peginterferon α-2a 180 μg once a week for 48 weeks and followed up for 24 weeks. The memory CD8^＋ lymphocytes were characterized by expressing CD45RA and CD27 markers. CD127 expression on cell surface was measured by four-colour flow cytometry. The difference of mean values between groups was evaluated by Mann-Whitney test. Results The CD127 expression on CD8^＋ T lymphocytes was significantly lower in HBeAg positive CHB patients compared to healthy controls （Z=2.889, P〈0.05）, which was negatively correlated with serum hepatitis B virus （HBV） DNA level and HBeAg titers. The CD127 expression increased along with the decrease of HBV DNA and HBeAg after 24-week, 48-week and 72-week treatment in patients showing good response to peginterferon α-2a, while CD127 expression didn＇t change markedly in non responders （Z24w = 1.954, Z48w = 2.789, Z72w = 2. 989; all P〈0. 05）. Conclusion CD127 expression on memory CD8^＋ lymphocytes increases along with effective anti-HBV treatment in CHB patients, which can be used as a marker for evaluating the effectiveness of anti-viral treatment.

关 键 词：CD8阳性T淋巴细胞 白细胞介素7受体α亚单位 聚乙烯二醇类 干扰素Α-2A 肝炎 乙型 慢性 肝炎e抗原 乙型 基因表达 

分 类 号：R686[医药卫生—骨科学]