胃肠道肿瘤细胞E2F-1转录因子活性对细胞周期的影响  

Activity of E2F-1 Transcriptional Factor in Cell Cycle of Gastrointestinal Cancer Cell Lines

作　　者：林彬[1] 周肖英[1] 程剑[1] 

机构地区：[1]无锡卫生高等职业技术学校内科教研室,214028

出　　处：《天津医药》2010年第10期891-892,929,共3页Tianjin Medical Journal

摘　　要：目的:探讨癌细胞E2F-1因子的表达状态及其与细胞周期的关系。方法:采用免疫荧光组织化学染色法检测8株胃肠道肿瘤细胞系E2F-1转录因子的表达,并采用流式细胞术检测肿瘤细胞周期的变化。结果:依据E2F-1阳性表达的程度不同,将8株肿瘤细胞系分为E2F-1高表达组(包括HGC-27、BGC-823、SGC-7901、HT29)和E2F-1低表达组(包括MKN45、MKN28、SH288、SW-620)。E2F-1高表达组G1/G0期、S期、细胞凋亡比例分别为(33.48±4.61)%、(63.91±5.02)%、(1.50±0.56)%,低表达组分别为(58.58±3.41)%、(35.38±4.03)%、(5.61±1.44)%,2组间比较差异有统计学意义(t分别为6.04、5.99和2.44,P<0.05或P<0.01)。结论:肿瘤细胞E2F-1因子的表达状态对细胞周期有明显的影响。E2F-1阳性的肿瘤细胞则倾向于更高的增殖速率。Objective：To investigate the activity of E2F-1 transcriptional factor in the cell cycle of gastrointestinal cancer cells.Methods：The expression of E2F-1 factor was detected immunohistochemically in 8 gastrointestinal cancer cell lines.The cell cycle was analyzed by flow cytometry in cancer cells.Results：The gastrointestinal cancer cells were divided into two groups,the E2F-1 high-expression group（including HGC-27,BGC-823,SGC-7901 and HT29） and E2F-1 low-expression group（including MKN45,MKN28,SH288 and SW-620）.The ratios of G1/G0 phase,S phase and apoptosis were（33.48± 4.61）%,（63.91±5.02）% and（1.50±0.56）% in high-expression group,and（58.58±3.41）%,（35.38±4.03）% and（5.61± 1.44）% in low-expression group.There were differences between these two groups.Conclusion：The status of E2F-1 expression has a great effect on cell cycle in cancer cells.The E2F-1-positive cancer cells tend to a higher proliferation rate.

关 键 词：胃肠肿瘤 E2F1转录因子 细胞周期 荧光免疫测定 流式细胞术 细胞系 肿瘤 细胞凋亡 

分 类 号：R979.1[医药卫生—药品]