吗啡对小鼠脑微血管内皮细胞P-糖蛋白表达的影响  

Effect of morphine on P-glycoprotein expression in mouse brain microvascular endothelial cells

作　　者：苏健[1] 阮祥才[1] 余守章[1] 许立新[1] 

机构地区：[1]广州医学院附属广州市第一人民医院麻醉科,510180

出　　处：《中华麻醉学杂志》2010年第6期700-702,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30700786）;广州市医药卫生重点项目（2007-ZDi-13）

摘　　要：目的 评价吗啡对小鼠脑微血管内皮细胞P-糖蛋白（P-gp）表达的影响.方法 小鼠脑微血管内皮细胞b.End3,培养于RPMI 1640无血清高糖培养基中,接种于10 cm培养皿中,分为3组,每组9皿,每皿2 ml,M组加入1 μg/ml吗啡,P＋M组在加入吗啡前1 h加入5 μnol/L NF-κB抑制剂吡咯二硫氨基甲酸酯,药物浓度均为终浓度,C组不作药物处理.加入吗啡后24 h时收集细胞,测定P-gp和NF-κB p65-abcb1b DNA复合体的表达水平.结果 与C组比较,M组P-gp和NF-κB p65-abcb1bDNA复合体的表达水平上调（P＜0.01）;与M组比较,P＋M组P-gp和NF-κB p65-abcb1b DNA复合体的表达水平下调（P＜0.05或0.01）.结论 吗啡可上调小鼠脑微血管内皮细胞P-gp表达,其机制与NF-κB介导的P-gp基因abcb1b激活有关.Objective To investigate the effect of morphine on P-glycoprotein （P-gp） expression in mouse brain microvascular endothelial cells. Methods The mouse brain microvascular endothelial cell line b. End3 was purchased from ATCC company （USA） and cultured at 37 ℃ in high glucose serum-free medium RPMI 1640 in 10 cm petri dishes and assigned to one of 3 groups（n = 9 each）： Ⅰ control group （group C）; Ⅱ morphine group （group M） and Ⅲ PDTC ＋ morphine group （group P ＋ M）. In group M the cells were exposed to morphine 1 μg/ml while in group P ＋ M the cells were pre-incubated with PDTC （NF-κB inhibitor） 5 μmol/L for 1 h before treatment with morphine. In group M and P＋ M after being treated with morphine 1 μg/ml for 24 h, the cells were collected for determination of P-gp expression and NF-κB p65-abcb1b protein-DNA binding analysis. Results P-gP expression and NF-κB p65-abcb1b protein-DNA binding were up-regulated in group M as compared with group C. The up-regulation was negated by pre-incubation with PDTC in group P ＋ M. Conclusion Morphine can induce up-regulation of the expression of endogenous P-gp in mouse brain microvascular endothelial cells by NF-κB mediated-abcb1 b gene activation.

关 键 词：吗啡 内皮 血管 糖蛋白类 

分 类 号：R285.5[医药卫生—中药学]