新城疫纳米乳疫苗研制及其免疫效力试验  

Preparation and Test of Newcastle Disease Vaccine Nanoemulsion

作　　者：陈建民[1] 欧阳五庆[1] 张旭[1] 胡帅[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《西北农业学报》2010年第9期34-37,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家科技支撑计划(2006BAD04A11);陕西省科技攻关项目(2008K02-01)

摘　　要：研制新城疫纳米乳疫苗,并考察其免疫效果。利用伪三元相图法优选配方,并考察其结构类型、形态粒径、稳定性、安全性及免疫效果。结果表明:新城疫纳米乳疫苗为W/O型,澄明均一,乳滴呈球形,平均粒径364 nm;稳定性良好;注射、口服接种安全;每只0.751、.0 mL免疫剂量可产生良好的免疫效果。新城疫纳米乳疫苗安全、稳定、免疫效果好,具有临床应用前景。To study the preparation and immunity test of Newcastle Disease Vaccine Nanoemulsion.Nanoemulsion was optimized by studying the pseudoternary phase diagrams,and its structural type,appearance,particle size,physical stability,safety and immune effect were tested respectively.The prepared nanoemulsion was the type of water-in-oil,transparent and homogeneous;Nanoemulsion was spherical under TEM;with the average diameter of 364 nm and stable;Nanoemulsion was safe to immunize by oral,injection vaccination;Nanoemulsion could produce better immune effect at 0.75,1.0 mL per chicken.The Newcastle Disease Vaccine Nanoemulsion has a promising clinical prospect.

关 键 词：新城疫 纳米乳 制备 抗体滴度 

分 类 号：R392.1[医药卫生—免疫学]