PolyI:C介导气道平滑肌细胞TLR3和IL-8、Eotaxin的表达  

Expression of TLR3 and IL-8,Eotax in in the airway smooth muscle cells induced by PolyI:C

作　　者：闫明华[1] 朱述阳[1] 段存玲 嵇桂娟[1] 

机构地区：[1]徐州医学院附属医院呼吸内科,江苏徐州221002

出　　处：《细胞与分子免疫学杂志》2010年第10期966-968,972,共4页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的:观察PolyI:C对大鼠气道平滑肌细胞(ASMCs)Toll样受体3(TLR3)及炎性细胞因子表达的影响,探讨ASMCs内TLR3表达与气道炎症间的关系。方法:体外培养大鼠ASMCs,传代培养后分为正常对照组和PolyI:C刺激组,PolyI:C刺激组又分为不同的时间点。用RT-PCR法检测细胞TLR3mRNA的表达;Western blot法检测ASMCs中核因子κB(NF-κB)的蛋白含量;ELISA法检测细胞培养上清Eotaxin和IL-8的浓度。结果:与正常对照组相比较,PolyI:C刺激组ASMCs内TLR3mRNA和NF-κB蛋白表达增加(P<0.05);ASMCs培养上清液中IL-8和Eotaxin浓度增加(P<0.05),并存在时间依赖性。结论:PolyI:C可以上调ASMCs内TLR3的表达,活化NF-κB,增加趋化因子Eotaxin和IL-8的浓度,参与哮喘的气道炎症反应。AIM：To observe the effect of PolyI：C on the expression of TLR3 and inflammation cytokine in the airway smooth muscle cells ASMCs of rat,to investigate relationship between the expression of Toll-like receptor 3 （TLR3） in the airway smooth muscle cells and airway inflammation.METHODS：Airway smooth muscle cells were derived from rat airway tissue and were cultured in vitro,ASMCs were divided into 2 groups：control group and PolyI：C group,PolyI：C group was divided into the different time spots.The mRNA expression of TLR3 was measured by RT-PCR.The level of NF-κB was measured by Western blot.The concertration of IL-8 and Eotaxin in ASMCs supernatant were determined by ELISA.RESULTS：The expression of TLR3 mRNA and NF-κB protein in the ASMCs in PolyI：C group was significantly higher than those in control group（P 0.05）,the concentration of IL-8 and Eotaxin in the ASMCs in PolyI：C group was significantly higher compared to control group （P〈0.05）;and have time dependent manner.CONCLUSION：Poly I：C promoted the expression of TLR3 in ASMCs,activated NF-κB,incresead the concentration of IL-8 and Eotaxin,involved in airway inflammation of asthma.

关 键 词：气道平滑肌细胞 PolyI:C TOLL样受体3 NF-ΚB 

分 类 号：R562[医药卫生—呼吸系统]