鸡传染性法氏囊病病毒超强株VP2基因重组新城疫病毒胚胎免疫安全性及效力试验  

Safety and efficacy of a recombinant Newcastle disease virus expressing the VP2 gene of very virulent infectious bursal disease virus

作　　者：王琪[1,2] 葛金英[2] 焦利红[1,2] 曹有才[2] 李晓艳[2] 马良[2] 马吉飞[1] 王笑梅[2] 步志高[2] 

机构地区：[1]天津农学院动物科学系,天津300384 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2010年第9期695-698,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家科技支撑计划(2006BAD06A16);国家科技支撑计划(2006BAD06A05)

摘　　要：为研制能够同时预防传染性法氏囊病(IBD)和新城疫(ND)的疫苗,本研究选用表达IBD病毒(IBDV)超强毒株(vvIBDV)VP2基因的重组ND病毒(NDV)LaSota疫苗株(rL-VP2),测定其半数鸡胚感染量、平均鸡胚致死时间、脑内接种指数和静脉内致病指数等指标,按不同剂量接种18胚龄SPF鸡胚,分别于出雏后第9d、第14d和第21d采血,用微量凝集法和ELISA方法测定血清中抗NDV抗体和抗IBDV抗体水平,并于出雏后28d用NDV强毒(F48E9株)和vvIBDVGx株攻毒,评估重组疫苗的胚胎免疫效果。结果显示:按104EID50/枚剂量进行免疫不会影响SPF鸡胚出雏率和出雏后21d存活率,并且该免疫组雏鸡能够对NDV强毒和vvIBDV攻毒提供安全的免疫保护。The recombinant Newcastle disease virus （NDV） （rL-VP2） expressing the VP2 gene of very virulent infectious bursal disease virus （vvIBDV） Gx strain as a candidate vaccine was evaluated. The vaccine was administered to 18 day old SPF chicken embryos with different doses and antibody titers were detected at 9,14 and 21 days post hatching. The results showed that the vaccine did not hamper hatching or surviving post hatching when rL-VP2 was administered with 104 EID50/egg dosage. The rL-VP2 vaccination could completely protect chickens against the NDV F48E9 strain and vvIBDV Gx strain challenge at four weeks post-hatch.

关 键 词：胚胎免疫 重组新城疫病毒 传染性法氏囊病毒超强毒株 VP2基因 

分 类 号：S852.4[农业科学—基础兽医学]