猪Toll样受体3、7和8基因的克隆与序列分析  

Cloning and sequencing analysis of porcine Toll-like receptor 3,7 and 8 genes

作　　者：张莉莉[1] 白娟[1] 王先炜[1] 李玉峰[1] 王兴龙[1] 姜平[1] 

机构地区：[1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏南京210095

出　　处：《中国预防兽医学报》2010年第9期727-730,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(30871868);国家重大转基因专项(2009ZX08009-143B);国家科技支撑计划(2007BAD86B02-3);教育部博士点基金项目(20090097120025)

摘　　要：为了对猪Toll样受体(TLR)3、7和8基因进行克隆与序列分析,本实验从猪肺泡巨噬细胞(PAM)中,利用RT-PCR方法分片段扩增猪TLR3、7、8基因,并克隆于pMD18-T载体中。根据测序结果分析,猪TLR3、7和8基因的ORF分别为2718bp、3153bp和3087bp,并分别编码906、1051和1029个氨基酸。同源性分析结果显示,它们与GenBank中登录的猪TLR的相应序列同源性达99%以上;与牛、马、羊和人的同源性较高,与鼠的同源性次之,与鸡的同源性最低,其蛋白分子结构预测表明猪TLR3、7、8均为跨膜蛋白。Toll-like receptors （TLR） were the key component of innate immune system. In the present study,porcine Toll-like receptor 3,7 and 8 （pTLR3,pTLR 7 and pTLR 8） genes were amplified by RT-PCR from porcine alveolar macrophages （PAM）. The PCR products were cloned and sequenced. The results showed that the pTLR3,pTLR7 and pTLR8 genes contained ORF of 2,718 bp,3,153 bp,3,087 bp in length that encoded 906 amino acids （aa）,1,051 aa and 1,029 aa,respectively. The nucleotide sequences of pTLR3,pTLR7 and pTLR8 shared 99% homology with the published pTLR sequences in GenBank. The structure prediction showed that the pTLR3,pTLR7 and pTLR8 were transmembrane proteins.

关 键 词：猪 TLR 克隆 序列分析 

分 类 号：Q786[生物学—分子生物学]