哈萨克绵羊MHC-DRB1基因SSCP多态性与细粒棘球蚴病遗传抗性关联分析  

Relationship between polymorphism of MHC-DRB1 gene and hydatidosis resistance in Kazak sheep

作　　者：温青娜[1] 贾斌[1] 申红[1] 白春生[1] 杜迎春[1] 李仁燕[1] 曾献存[1] 周其伟[1] 田永芝[1] 吕利民[1] 惠文巧[1] 

机构地区：[1]新疆石河子大学动物科技学院,石河子832003

出　　处：《中国人兽共患病学报》2010年第9期805-809,共5页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助项目(30660124)

摘　　要：目的研究新疆哈萨克绵羊MHC-DRB1基因多态性与细粒棘球蚴病遗传抗性之间的相关性。方法应用巢式PCR-SSCP对细粒棘球蚴病阳性和阴性各100只哈萨克绵羊的MHC-DRB1第2外显子进行多态性分析。结果试验结果共检测出15个等位基因和37种基因型。χ2适合性检验结果表明,哈萨克绵羊MHC-DRB1基因第2外显子的SSCP带型未达到Hardy-Weinberg平衡(P<0.01)。将细粒棘球蚴病阴性和阳性哈萨克绵羊的等位基因频率和基因型频率进行差异性显著检验和相对危险性估计(RR值估计)分析,发现等位基因H(P<0.01,RR=0.394)和F(P<0.01,RR=0.465)、基因型FF(P<0.05,0.352)和GH(P<0.05,RR=0.45)与细粒棘球蚴病的抗性具有较强的相关性;等位基因B(P<0.01,RR=14.5>4.1)和基因型GG(P<0.01,RR=13.5>4.1)与细粒棘球蚴病易感性具有很强的相关性,K(P<0.01,RR=3.76)和G(P<0.01,RR=3.395)与细粒棘球蚴病易感性具有较强的相关性,基因型KK(P<0.05,RR=3.996)与细粒棘球蚴病易感性具有较强的相关性。结论巢式PCR-SSCP方法证明了哈萨克绵羊MHC-DRB1基因第2外显子存在丰富的多态性,并初步筛选出了与包虫病抗性或易感性相关的等位基因与基因型。To analyze genetic correlations between polymorphism of MHC-DRB1 gene and hydatidosis resistance in Kazak sheep,the polymorphism of exon 2 from MHC-DRB1 in 100 healthy and 100 infected sheep were detected by nested PCR SSCP.Results showed that the extensive polymorphism of exon 2 from MHC-DRB1 was detected and there were 37 genotypes controlled by 15 alleles.Results of χ2 test showed that patterns of MHC-DRB1 were significantly deviant from Hardy-Weinberg equilibrium（P0.01）.By comparing alleles and genotypic frequencies of MHC-DRB1 in healthy Kazak sheep with those in infected ones,and to analyze the relative risk（RR） value,allele H（P0.01,RR=0.394） and F（P0.01,RR=0.465）,as well as genotype FF（P0.05,0.352） and GH（P0.05,RR=0.45）,were proved to have strong correlation with hydatidosis resistance.Allele B（P0.01,RR=14.54.1） and genotype GG（P0.01,RR=13.5） have the strongest predisposition to hydatidosis.And allele K（P0.01,RR=3.76） and G（P0.01,RR=3.395）,as well as genotype KK（P0.05,RR=3.996）,also has stronger predisposition to hydatidosis.Application of nested PCR-SSCP method confirmed the extensive polymorphism of Kazak sheep MHC-DRB1 genes and reported that the alleles and genotypes were relevant to the resistance or susceptibility of echinococcosis in Kazak sheep.

关 键 词：哈萨克绵羊 MHC-DRB1 PCR-SSCP多态性 细粒棘球蚴病 

分 类 号：R383.3[医药卫生—医学寄生虫学]