空肠弯曲菌鞭毛蛋白单克隆抗体的制备  

Development of monoclonal antibody against flagellin of Campylobacter jejuni

作　　者：唐泰山[1,2] 王婷[1,2] 祝长青[1] 蒋原[1] 姚火春[2] 张常印[1] 薛峰[1] 栾军[1] 

机构地区：[1]江苏出入境检验检疫局,南京210001 [2]南京农业大学动物医学院,南京210095

出　　处：《中国人兽共患病学报》2010年第9期843-846,共4页Chinese Journal of Zoonoses

基　　金：国家质检总局科研项目(No.2008IK19)

摘　　要：目的为获得空肠弯曲菌鞭毛蛋白单克隆抗体,用于空肠弯曲菌的检测和研究。方法用空肠弯曲菌ATCC29428株灭活全菌作为抗原,免疫BALB/c小鼠,待抗体水平达到1∶51 200时,取脾细胞与生长良好的对数期Sp2/0进行融合。同时以粗提天然鞭毛蛋白和人工表达重组鞭毛蛋白包板,用间接ELISA方法筛选,多次克隆化培养后获得的单抗用Western blot进行生物学特性鉴定。结果获得3株持续、稳定分泌抗空肠弯曲菌鞭毛蛋白单克隆抗体的杂交瘤细胞株,分别命名为1G8、1G9和3F2。结论获得的空肠弯曲菌鞭毛蛋白单克隆抗体为建立空肠弯曲菌的蛋白检测及后续研究奠定基础。To prepare monoclonal antibodies（McAbs） against Campylobacter jejuni（C.jejuni）,BALB/c mice were immunized with inactivated C.jejuni ATCC 29428 cells.After fusing and cloning,the hybridoma cell lines secreted McAbs against C.jejuni flagellin were screened by regular cell fusion and subcloning approach.The specificities of these McAbs were determined by ELISA and Western blotting.Results suggested that three hybridoma cell lines secreted McAbs against C.jejuni flagellin are produced and designated as 1G8,1G9 and 3F2.

关 键 词：空肠弯曲菌 鞭毛蛋白 杂交瘤 单克隆抗体 

分 类 号：R392.2[医药卫生—免疫学]