钙网蛋白calreticulin在小鼠睾丸中的表达及意义  

Characterization of calreticulin in adult mouse testis

作　　者：卢秀真[1,2] 吴亦波[1] 周作民[1] 黄晓燕[1] 

机构地区：[1]南京医科大学生殖医学实验室,江苏南京210029 [2]镇江卫生学校基础教研室,江苏镇江212004

出　　处：《南京医科大学学报（自然科学版）》2010年第9期1219-1222,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助(30700274)

摘　　要：目的:研究钙网蛋白(calreticulin,CRT)在成年小鼠睾丸组织中的表达及分布,探讨CRT在小鼠睾丸发育及其精子发生过程的功能。方法:运用免疫印迹法和免疫组织化学术,检测CRT在小鼠睾丸组织中的表达和分布。结果:免疫印记法证实CRT在成年小鼠睾丸高表达,同时免疫组织化学显示阳性信号主要集中在小鼠睾丸精原细胞和间质细胞。结论:CRT在小鼠睾丸中的定位提示其可能与精原细胞更新和分化以及雄激素分泌密切相关。Objective：To investigate expression and distribution of calreticulin （CRT） in the adult mouse testis and explore the preliminary function of CRT during testes development and spermatogenesis. Methods：The expression and distribution of calreticulin （CRT） in the adult mouse testis were detected by western blotting （WB） and Immunohistochemistry （IHC）. Results：Western blotting result showed CRT expressed highly in mouse testis. Immunohistochemistry result revealed positive signals were widely distributed while strong signals were mainly in primary spemlatogonia and Leydig cells. Conelusion：CRT expressed highly in adult mouse testis, and it may be involved in spermatogonia self-renew/differentiation and testosterone secretion mechanisms.

关 键 词：钙网蛋白 小鼠睾丸 免疫印记法 免疫组织化学 

分 类 号：R392.11[医药卫生—免疫学]