莱菔硫烷通过线粒体诱导HepG-2细胞凋亡  

Study on apoptotic effect induced by sulforaphane in HepG-2 cells via mitochondrial pathway

作　　者：季宇彬[1,2,3] 高鹏[1,2] 邹翔[1,2] 孙胜男[1,2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,哈尔滨150076 [3]哈尔滨商业大学药物所博士后科研工作站,哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2009年第6期641-644,共4页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家自然科学基金项目(30300284);黑龙江省自然科学基金项目(D200802);黑龙江省骨干教师项目(1154G36)

摘　　要：探讨莱菔硫烷(SFN)通过线粒体途径诱导HepG-2细胞凋亡的机制.不同剂量SFN处理体外培养的HepG-2细胞株48h,流式细胞仪检测SFN对HepG-2细胞凋亡率影响;采用Western Blot法测定SFN对HepG-2细胞Cyt-c蛋白表达的影响;酶活性检测试剂盒测定SFN对HepG-2细胞内caspase-3和caspase-9酶活性的影响.SFN能够有效地诱导HepG-2细胞凋亡,10、20、40μmol/L的SFN作用于HepG-2细胞48h后,与对照组比较凋亡率显著升高(P<0.01),并呈剂量依赖性;SFN可使HepG-2细胞胞浆中Cyt-c蛋白含量升高,与对照组比较差异显著(P<0.05);同时使细胞内caspase-3和caspase-9的活性升高,并呈一定的剂量依赖性.SFN可诱导人肝癌HepG-2细胞的凋亡,可通过促进Cyt-c的释放,进而激活caspase-9,再激活下游caspase-3,最终引起细胞凋亡.To investigate the mechanism of inducing apoptosis effect through the mitochondrial pathway in HepG-2 by sulforaphane （SFN）. HepG-2 cells are treated with different concentration of SFN in vitro for 48 hours. Flow cytometry is used to detect apoptosis rate of SFN on HepG-2,Western Blot is used to detect the expression of Cyt-c protein in HepG-2,and the activity detection kits are employed to examine the activity of caspase-3 and caspase-9. SFN could effectively induce cell apoptosis of HepG-2 and the apoptosis rates are increased in a dose-dependent manner（P0.01）. SFN could also remarkably up-regulate the expression of Cyt-c protein in HepG-2 cells （P0.05）. Meanwhile,the activity of caspase-3 and caspase-9 is increased which showed a dose-dependent manner. SFN can induce apoptosis of human hepatoma HepG-2 cells,and activating the mitochondrial apoptotic pathway is one of the main mechanism of SFN.

关 键 词：莱菔硫烷 HEPG-2细胞 细胞凋亡 

分 类 号：R285[医药卫生—中药学]