钙和NO对NaCl胁迫下黄瓜幼苗生长和活性氧代谢的影响  

Effects of Ca^(2+) and nitric oxide on growth and active oxygen metabolism of cucumber seedlings under NaCl stress

作　　者：樊怀福[1,2] 杜长霞[1] 郭世荣[1] 李娟[1] 

机构地区：[1]南京农业大学园艺学院,农业部南方蔬菜遗传改良重点开放实验室,江苏南京210095 [2]浙江农林大学农业与食品科学学院,浙江临安311300

出　　处：《植物营养与肥料学报》2010年第5期1224-1231,共8页Journal of Plant Nutrition and Fertilizers

基　　金：国家重点基础研究发展计划(2009CB119000);现代农业产业技术体系建设专项资金;国家科技支撑计划(2008BADA6B07);江苏省农业三项工程[SX(2008)026];国家自然科学基金项目(30871736、30900995)资助

摘　　要：采用营养液培养的方法,研究了Ca2+对外源一氧化氮(Nitric oxide,NO)所诱导的NaCl胁迫下黄瓜幼苗生长、活性氧代谢的影响。结果表明,添加外源NO或Ca2+显著缓解了NaCl胁迫对黄瓜幼苗生长的抑制,叶片和根系超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性较单独NaCl胁迫处理显著提高,丙二醛(MDA)和过氧化氢(H2O2)的含量、超氧阴离子(O.-2)产生速率明显下降;添加NO的同时添加Ca2+通道抑制剂La3+抑制了NO的这些调节作用。结果表明Ca2+对NO诱导的NaCl胁迫下黄瓜幼苗植株活性氧清除能力的提高起重要作用,NO的作用可能依赖于胞浆Ca2+。The study was conducted in a nutrient solution to investigate the effects of Ca^2＋ on growth and active oxygen metabolism of cucumber seedlings induced by exogenous nitric oxide under the 50 mmol/L NaCl stress.The results show that plasma membrane Ca^2＋ channel blockers La3＋ block the effects on growth and active oxygen metabolism induced by exogenous nitric oxide.Exogenous nitric oxide or Ca^2＋ alleviate the injury to seedlings under the 50 mmol/L NaCl stress significantly,and the seedling growth,activities of SOD,POD and CAT are increased and the rate of O2^-· production,contents of MDA and H2O2 are decreased.These results indicate the La^3＋ blocks those effects induced by exogenous nitric oxide under the NaCl stress,and Ca^2＋ might play an important role in growth and active oxygen metabolism induced by exogenous nitric oxide.The effect of nitric oxide here depends on the content of Ca^2＋ in cytoplasm.

关 键 词：一氧化氮 NACL胁迫 黄瓜幼苗 活性氧 氯化镧 

分 类 号：S642.2[农业科学—蔬菜学]