SNP对NaHCO_3胁迫下黄瓜幼苗生长及氮代谢酶活性的影响  

Effects of SNP on the growth and nitrogen metabolism enzymes activities of cucumber seedlings under NaHCO_3 stress

作　　者：林燕[1] 洪艳艳[1] 史庆华[1,2] 王秀峰[1,2] 魏珉[1,2] 杨凤娟[1,2] 

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018 [2]作物生物学国家重点实验室,山东泰安271018

出　　处：《植物营养与肥料学报》2010年第5期1294-1298,共5页Journal of Plant Nutrition and Fertilizers

基　　金：国家自然科学基金项目(30800751)资助

摘　　要：采用溶液培养方法,研究了外源NO供体硝普钠(sodium nitroprusside,SNP)对NaHCO3胁迫下黄瓜幼苗的缓解效应。结果表明,100μmol/L SNP能有效减轻30 mmol/L NaHCO3对黄瓜植株地上部和地下部生长的抑制,提高了NaHCO3胁迫下黄瓜叶片叶绿素和类胡萝卜素含量、净光合速率(Pn)以及荧光参数Fv/Fm和ΦPSⅡ。HaHCO3胁迫显著抑制了氮代谢相关酶硝酸还原酶(NR)、谷氨酰胺合成酶(GS)和谷氨酸合成酶(GOGAT)的活性;外加SNP处理明显缓解了NaHCO3对它们活性的抑制。A solution culture experiment was conducted to study the effects of SNP on plant growth,photosynthesis,antioxidant enzyme activity and nitrogen metabolism in cucumber seedlings under NaHCO3 stress.The results indicated that 100 μmol/L SNP promoted the growth of cucumber seedlings and increased chlorophyll and carotenoid contents,net Photosynthetic rate（Pn）,Fv/Fm and ΦPSⅡunder NaHCO3 stress.Compared with control,the activities of nitrate reductase（NR）,glutanine synthetase（GS） and glutamate synthase（GOGAT） were significantly inhibited by NaHCO3 stress and SNP increased their activities.

关 键 词：黄瓜 SNP NAHCO3胁迫 酶活性 

分 类 号：S642.2[农业科学—蔬菜学] S482.8[农业科学—园艺学]