花生(Arachis hypogea L.)体细胞胚胚性特异蛋白的研究  

Study on Markey Proteins for Somatic Embryogenesis in Mature Peanut( Arachis hypogea L. )

作　　者：肖显华[1] 王庆华[1] 林荣双[1] 王顺珍[1] 

机构地区：[1]烟台大学生物化学系

出　　处：《烟台大学学报（自然科学与工程版）》1999年第3期185-188,共4页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：山东省自然科学基金

摘　　要：花生萌发３ｄ的胚叶在诱导培养基上出现高频率的体细胞胚发生．ＳＤＳ－ＰＡＧＥ分析结果表明：在体细胞胚发生过程中，诱导第三天开始出现３条新的蛋白谱带，７ｄ后出现２条新带，１５ｄ后又出现３条；而在非胚性组织中检测到抑制性的特异蛋白条带．A high frequency somatic embryogenesis system is initiated by culturing 3 day old leaflets of mature peanut on MS induced medium. SDS polyarcylamide gel electrophoresis (SDSPAGE) is employed to examine the soluble proteins of somatic embryos. The results provide that three new proteins are detected after 3d culturing, two special proteins are found after 7d and three new protein bands are detected in the electrophoresis gram. Moreover, two special proteins are found in the non embryogenic tissue.

关 键 词：体细胞胚 胚性特异蛋白 花生 组织培养 

分 类 号：S565.201[农业科学—作物学]