血清MMP-2及其组织抑制因子-1与糖尿病足的关系  

Association of serum metalloproteinase-2 and tissue inhibitors-1 with diabetic foot

作　　者：李志红[1] 房辉[1,2] 张云良[3] 郭淑琴[3] 甄艳凤[1] 

机构地区：[1]河北医科大学内科教研室,石家庄050017 [2]唐山市工人医院内分泌二科,河北唐山063000 [3]保定市第一中心医院内分泌科,河北保定071000

出　　处：《临床检验杂志》2010年第5期366-368,共3页Chinese Journal of Clinical Laboratory Science

摘　　要：目的评价血清基质金属蛋白酶-2(MMP-2)及其组织抑制因子-1(TIMP-1)与糖尿病足慢性溃疡的关系及两指标的相关性。方法 ELISA法测定糖尿病足(DF组)患者78例、2型糖尿病(T2DM)非糖尿病足(NDF组)患者111例和健康体检者(CON组)150例血清MMP-2、TIMP-1水平。结果 CON、NDF和DF三组血清MMP-2水平依次升高,差异有统计学意义(P<0.05);DF、NDF组血清TIMP-1水平均低于CON组(P<0.05),DF、NDF组间差异无统计学意义;DF组MMP-2/TIMP-1的比值高于NDF和CON组,NDF与CON组间差异无统计学意义。血清TIMP-1与MMP-2呈高度负相关(r=-0.852,P<0.05)。Logistic回归分析示,MMP-2是DF的独立危险因素。结论血清MMP-2升高是糖尿病患者发生DF的独立危险因素,与TIMP-1负相关。糖尿病患者血清MMP-2水平升高对于预测DF发生有一定价值。Objective To evaluate the association between serum matrix metalloproteinase-2 （MMP-2）,tissue inhibitors of matrix metalloproteinase-1 （TIMP-1） levels and diabetic foot ulcer.Methods The levels of MMP-2 and TIMP-1 in sera from 78 patients with diabetic foot ulcers （DF group） and 111 diabetes patients without diabetic foot （NDF group） were determined by ELISA,and 150 healthy subjects were enrolled as controls.Results The levels of MMP-2 in DF groups [（81.7 ± 48.8） μg/L] and NDF groups [（46.1 ±18.2） μg/L]was significantly higher than that [（34.4 ±16.4） μg/L]in controls （P〈0.05） .The levels of TIMP-1 in both DF and NDF groups were lower than that of controls （P〈0.05）,but there was no statistical difference between the 2 groups （P〈0.05） .The radio of MMP-2 /TIMP-1 was higher in DF groups than those in NDF and controls,and there was no difference between NDF and controls groups （P〈0.05） .Regression analysis showed a significant negative correlation between serum TIMP-1 and MMP2 （r =-0.852,P〈0.05）,and MMP-2 was an independent predictor of DF.Conclusion Elevated serum MMP-2 level appears to be an important risk factor for diabetic foot showing negative correlation with TIMP-1.Serum MMP-2 in the patients with diabetes may be diagnostically valuable for predict DF.

关 键 词：糖尿病足 基质金属蛋白酶-2 基质金属蛋白酶组织抑制因子-1 

分 类 号：R587.2[医药卫生—内分泌]