GRIM-19对子宫颈癌细胞株HeLa细胞Survivin基因表达的影响  

Effect of GRIM-19 on expression of Survivin gene in cervical cancer cell line HeLa cells

作　　者：卫莹[1] 凌斌[1] 朱靖[1] 李敏[1] 范少君[1] 冯定庆[1] 肖卫华[2] 周颖[1] 

机构地区：[1]安徽医科大学附属省立医院妇产科分子实验室安徽省分子医学重点实验室 [2]中国科技大学生命科学院免疫学研究所

出　　处：《中国妇幼保健》2010年第29期4274-4276,共3页Maternal and Child Health Care of China

基　　金：安徽省自然科学基金项目(090413117);安徽省高等学校省级自然科学研究项目(KJ2010B375)

摘　　要：目的:探讨稳定转染GRIM-19对子宫颈癌细胞株HeLa细胞凋亡抑制基因Survivin的表达以及细胞增殖的影响。方法:采用脂质体转染法将GRIM-19重组质粒转染子宫颈癌细胞株HeLa细胞,建立稳定表达GRIM-19的HeLa细胞株,采用RT-PCR检测HeLa细胞转染GRIM-19后mRNA水平表达情况,采用WesternBlot检测稳定转染后GRIM-19与Survivin的表达情况,应用细胞计数法观察各组细胞增殖水平。结果:建立稳定的GRIM-19基因表达的HeLa细胞株,命名为HeLa/G19细胞,对照组细胞命名为HeLa/Con细胞。RT-PCR检测显示GRIM-19mRNA表达明显增加,WesternBlot检测显示GRIM-19的蛋白表达显著升高、Survivin蛋白的表达水平显著降低。细胞计数法实验显示HeLa/GRIM-19细胞增殖较HeLa/Con细胞明显减慢(P<0.05)。结论:GRIM-19可以抑制子宫颈癌细胞Survivin的表达,阻滞细胞的生长,可能是子宫颈癌治疗的新靶点。Objective：To explore the effects of GRIM-19 on expression of Survivin gene in cervical cancer cell line HeLa cells and cell proliferation.Methods：The cancer cell line HeLa cells were transferred with GRIM-19 recombinant plasmid by liposome infection protocol,HeLa cells expressing GRIM-19 stably were constructed,RT-PCR was used to detect the expression level of GRIM-19 mRNA after transfection,Western blot was used to detect the expression levels of GRIM-19 and Survivin after transfection,cytometry was used to observe the proliferative levels of cells in different groups.Results：Stable HeLa cell line expressing GRIM-19 gene was established and named as HeLa/G19 cells,for control group,the cell line was named as HeLa/Con cells;the results of RT-PCR indicated that the expression of GRIM-19 mRNA increased significantly,the results of Western blot indicated that the expression of GRIM-19 protein increased significantly,while the expression of Survivin protein decreased significantly;the results of cytometry indicated that the proliferation of HeLa/GRIM-19 cells was slower than that of HeLa/Con cells （P 0.05）.Conclusion：GRIM-19 can inhibit the expression of Survivin in cervical cancer cells and block cell proliferation,which may be a new target for treatment of cervical cancer.

关 键 词：GRIM-19 转染 子宫颈癌 SURVIVIN 

分 类 号：R711.74[医药卫生—妇产科学]