农杆菌介导法将hbFGF基因导入大豆的研究  

Study on cloning hbFGF gene into soybean by Agrobacterium tumefaciens

作　　者：于凤丽[1] 陈伟莉[1] 

机构地区：[1]黑龙江省黑河学院,黑龙江黑河164300

出　　处：《大豆科技》2010年第5期3-6,共4页Soybean Science & Technology

基　　金：黑龙江省教育厅科学技术研究项目(11533044)

摘　　要：本研究将hbFGF基因克隆到质粒pCambia1301载体,利用基因重组技术在大豆毛状根中表达。以大豆品种吉农13的子叶节和胚轴为外植体,通过发根农杆菌C58C1介导进行转化。得到的毛状根,通过潮霉素抗性筛选,经PCR检测和Western blot印迹分析,结果表明:阳性毛状根中整合并表达了hbFGF基因。In this study,hbFGF gene was cloned into plasmid vector pCambia1301,by using the recombinant expression in soybean hairy roots.Taking Jinong13 soybean varieties,Jinong13 cotyledonary node and hypocotyl as explants,by using Agrobacterium rhizogenes C58C1 rhizogenes-mediated transformation,the experiment obtained hairy roots,which used the hygromycin resistance selection,by PCR detection and Western blot analysis.The results showed that：positive hairy roots in the integration and expression of hbFGF genes.

关 键 词：大豆 农杆菌 hbFGF基因 

分 类 号：Q503[生物学—生物化学]