骨骼肌膜DNA结合蛋白  

DNA Binding Protein in Skeletal Muscle Membrane

作　　者：陈光慧[1] 张晨晖[1] 秦阳军 汤健[1] 赵文贾 

机构地区：[1]北京医科大学心血管基础所 [2]北京医科大学生物化学与分子生物学系

出　　处：《高技术通讯》1999年第5期49-52,共4页Chinese High Technology Letters

基　　金：863计划资助项目

摘　　要：应用差速及蔗糖密度梯度离心法提取了骨骼肌非核的肌膜蛋白，发现在肌膜上有一种ＤＮＡ结合蛋白质，其分子量为２９ｋＤａ，能与环状质粒ＤＮＡ和双链线状ＤＮＡ发生特异性结合。With differential and sucrose density gradient centrifugations, total rat nonnuclear sarcoplasmic membranes were purified. 32 P DNA overlay technique revealed the presence of a 29kDa protein that bounds closed circular (cc) and linearized(L) double stranded DNA specifically in total rat sarcoplasmic membranes. The DNA binding activities could be completely inhibited by the presence of unlabled CC or L double stranded DNA. The membrane associated DNA binding protein may involve in the skeletal muscle mediated gene transfer.

关 键 词：基因转移 骨骼肌 DNA 基因治疗 肌膜蛋白 

分 类 号：Q785[生物学—分子生物学]