人参皂苷Rg1对血管紧张素Ⅱ诱导人脐静脉内皮细胞凋亡的影响  

Reversal effects of ginsenoside Rg1 on apoptosis of human umbilical vein endothelial cells induced by AngⅡ

作　　者：贺国洋[1] 李巍[2] 焦红丽[3] 千新来[1] 

机构地区：[1]新乡医学院病理学教研室,河南453003 [2]新乡医学院第二附属医院中心实验室,河南453003 [3]新乡医学院组织胚胎学教研室,河南453003

出　　处：《重庆医学》2010年第19期2581-2582,2585,共3页Chongqing medicine

摘　　要：目的探讨人参皂苷Rg1对血管紧张素Ⅱ(AngⅡ)诱导人脐静脉内皮细胞(HUVECs)凋亡的逆转作用。方法体外培养HUVECs,采用台盼蓝染色方法筛选人参皂苷Rg1最适浓度,采用琼脂糖凝胶电泳和末端脱氧核糖核苷酸转移酶介导的缺口末端标记法(TUNEL)染色检测细胞凋亡情况。结果台盼蓝染色显示人参皂苷Rg1的最适浓度为40μg/mL。琼脂糖凝胶电泳显示,对照组和Rg1组未见凋亡条带,AngⅡ组可见清楚的细胞凋亡的"梯状"条带,而AngⅡ+Rg1组可见不明显的细胞凋亡的"梯状"DNA断裂条带。TUNEL染色显示,与对照组和Rg1组相比,AngⅡ组细胞凋亡数量增加,差异有统计学意义(P<0.01);与AngⅡ组相比,AngⅡ+Rg1组细胞凋亡数量减少,差异有统计学意义(P<0.01),凋亡百分比由38.667%下降到10.667%,但仍高于对照组和Rg1组(P<0.01)。结论 40μg/mL人参皂苷Rg1可一定程度逆转AngⅡ诱导的HUVECs凋亡。Objective To explore reversal effects of ginsenoside Rg1 on apoptosis of human umbilical vein endothelial cells（HUVECs）induced by angiotensinⅡ（AngⅡ）.Methods The HUVECs were cultured in vitro.The optimal concentration of ginsenoside Rg1 was screened by staining of trypan blue.The apoptosis of cells were detected by agarose gel electrophoresis and terminal deoxynucleotidyl transferse-mediated dUTP nick end labeling（TUNEL）staining.Results The result from staining of trypan blue showed that the optimal concentration of ginsenoside Rg1 was 40 μg/mL.The results from agarose gel electrophoresis indicated that there were no apoptotic ladder in the control group and Rg1 group,but apoptotic ladder was observed clearly in the group of AngⅡ,and obsolete apoptotic ladder were observed in the group of AngⅡ＋Rg1.The results from the TUNEL staining displayed that compared with the control group and Rg1 group,the quantity of apoptotic cells in AngⅡgroup increased significantly（P0.01）;Compared with the group of AngⅡ,the quantity of apoptotic cells in the group of AngⅡ＋Rg1 decreased markedly（P0.01）,and the apoptotic percentage decreased from 38.667% to 10.667%,but the percentage aslo higher than the control group and Rg1 group.Conclusion The partial reverse effects of ginsenoside Rg1 on the apoptosis of HUVECs induced by AngⅡ are identified.

关 键 词：人参皂苷RG1 血管紧张素Ⅱ 人脐静脉内皮细胞 凋亡 

分 类 号：R285[医药卫生—中药学]