有机磷水解酶全细胞生物催化剂的特性  

The Characterization of Organophosphorus Hydrolase Whole-Cell Biocatalyst

作　　者：黎小军[1] 林陈水[2] 

机构地区：[1]新余学院生化教研室,江西新余338000 [2]浙江工业大学药学院,浙江杭州310032

出　　处：《江西师范大学学报（自然科学版）》2010年第4期397-400,共4页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：江西省教育厅青年科学基金(GJJ09625)资助项目

摘　　要：利用有机磷水解酶大肠杆菌细胞表面展示工程菌为全细胞催化剂,研究了其降解对氧磷的反应.确定了最适反应条件为:反应温度37℃、pH值8.0、底物浓度2.0 mmol/L、振荡速率120 r/min,在此条件下反应5 h,对氧磷降解率可达80%左右,且全细胞催化剂具有较好的稳定性和可重复使用性,显示了良好的应用前景.Degrading paraoxon by whole-cell organophosphorus hydrolase that express by cell-surface display E.coli engineering strain was studied.The optimal reaction conditions for the biocatalysis are 37 ℃ of temperature,pH 8.0,2.0 mmol/L of substrate concentration and 120 r/min of shaking rate.Under these optimized conditions,about 80% of paraoxon was degraded after 5 h reaction.The whole-cell enzyme has a good reusability and a high stability,which demonstrates the prospect for real application.

关 键 词：有机磷水解酶 全细胞生物催化剂 降解 特性 

分 类 号：Q814[生物学—生物工程]