新型含苯硼酸和喹啉的囊泡荧光多糖传感器的制备  

Preparation of the Fluorescent Vesicular Sensor Containing both Phenylboronic Acid and Quinolinium for Saccharide

作　　者：李广全 谢昕 邵月君 巨娟侠 慕雪梅 

机构地区：[1]中国石油兰州化工研究中心,甘肃兰州730060

出　　处：《化学研究》2010年第5期58-62,共5页Chemical Research

摘　　要：利用合成的含有识别基团苯硼酸和荧光读出基团喹啉的新型双亲化合物对硼酸苯甲基-8-十六烷氧基溴化喹啉(BHQB)在水中自组织成囊泡,囊泡的相变温度为52.4℃;当向囊泡体系加糖时,BHQB囊泡中的喹啉生色基在508nm的荧光峰强度急剧减弱,425nm处荧光逐渐增强.荧光强度变化可能归于所形成的硼酸酯改变了双亲化合物中硼原子的杂化轨道形式,进一步引起了整个分子内部的电子云排布所致.BHQB囊泡与糖的相互作用而导致体系荧光强度变化,并且这种变化的幅度与加入糖的种类和量均有关.因此体系有可能应用于检测生物物质如糖的化学传感器.A novel amphiphile containing two functional groups of both quinolinium and phenylboronic acid,N-（boronobenzyl）-8-hexadecyloxyquinolinium bromide（BHQB）was prepared.The amphiphile has self-organized into vesicles in the selective solution of water.The Tc of the vesicles from crystal to liquid crystal is 52.4 ℃.Addition of saccharide to the vesicular solution,the fluorescent intensities of quinolinium in BHQB vesicles centred at 425 nm increased and that at 508 nm decreased dramatically.The change of fluorescence intensity is ascribed to boronic ester formation which might change the boron atom hybrid orbital forms further causing the entire molecular electron cloud arrangement.The vesicular sensor through the interactions between boronic acid and saccharide displayed at a biomimetic membrane interface can be detected by means of fluorescence intensities.The results suggest that the fluorescent vesicles might be used as biosensors to determine saccharide.

关 键 词：双亲化合物 传感器 囊泡 糖识别 荧光光谱 

分 类 号：O657.3[理学—分析化学]