薄层荧光扫描法测定花生根中白藜芦醇的含量  

Determination of Resveratrol in Peanut Root by Thin Layer Chromatography Fluorescence Scanning

作　　者：韩小丽 张士真 呼亚旭[3] 李明静[3] 

机构地区：[1]河南省医药学校,河南开封475001 [2]商丘市技师学院,河南商丘476000 [3]河南大学化学化工学院,河南开封475004

出　　处：《化学研究》2010年第5期88-89,96,共3页Chemical Research

摘　　要：在硅胶G铝箔板上,以V(氯仿):V(丙酮):V(甲酸)=8:1:0.2的溶液为展开剂,狭缝尺寸为3mm×0.45mm,检测波长为335nm,采用薄层荧光扫描法测定花生根中白藜芦醇的含量.结果表明:白藜芦醇在21.6～108.0ng范围内线性关系良好,回归方程为Y=-664.701+12.666X,R=0.9969,花生根中白藜芦醇的含量为358.89μg/g.The content of resveratrol was determined by thin layer chromatography fluorescence scanning on silica-gel G aluminium foil plate.The developing solvent was composed by chloroform,acetone and formic acid（8：1：0.2）.The slit size was 3 mm×0.45 mm and the detection wavelength was 335 nm.The results showed that the calibration curves were linear in the range of 21.6～108.0 ng （R = 0.996 9）,and the content of resveratrol in peanut root was 358.89 μg/g.

关 键 词：薄层荧光扫描法 白藜芦醇 花生根 

分 类 号：O652.63[理学—分析化学]