杜氏赖草(Leymus duthiei)及杜氏赖草长芒变种(Leymus duthiei var. longearistatus)的nrDNA ITS序列分析  

Sequence Analysis of the ITS Region of Nuclear Ribosomal DNA (nrDNA) in Leymus duthiei and Leymus duthiei var. longearistatus

作　　者：凡星[1] 廖莎[1] 罗春莲[1] 康厚扬[1] 张海琴[1] 周永红[1] 

机构地区：[1]四川农业大学小麦研究所,四川温江611130

出　　处：《四川农业大学学报》2010年第3期265-270,共6页Journal of Sichuan Agricultural University

基　　金：国家自然科学基金(30870154;30900087);四川省教育厅项目

摘　　要：本研究对杜氏赖草(Leymus duthiei)及杜氏赖草长芒变种(Leymus duthiei var.longearistatus)的nrDNA ITS进行多克隆测序,探讨ITS序列在其个体内的多样性和个体间的遗传分化。序列多态性、基因谱系和rRNA基因二级结构分析表明:①由于致同进化作用,ITS序列在L. duthiei和L.duthiei var. longearistatus中可能朝Ns基因组一方发生偏向;③致同进化的驱动力可能促使不同的ITS拷贝序列在L.duthiei var. longearistatus中比在L. duthiei中更趋于一致;③ITS序列在L.duthiei和L. duthiei var. longearistatus中的遗传分化可能与它们的地理隔离有关。Sequencing of multiple clones of ribosomal ITS sequences in Leymus duthiei and Leymus duthiei var.longearistatus were performed to estimate ITS polymorphism within and between individuals.Sequence diversity patterns,ITS RNA secondary structure and genealogical analysis suggested that： ①due to concerted evolution,ribosomal ITS sequences in L.duthiei and L.duthiei var.longearistatus might be biased towards the Ns genome donor;②concerted evolution might help to make the ribosomal ITS sequences within L.duthiei var.longearistatus more homogenized than that within L.duthiei;③ITS sequence divergence between L.duthiei and L.duthiei var.longearistatus might be responsible for their geographic isolation.

关 键 词：杜氏赖草 杜氏赖草长芒变种 NRDNA ITS 致同进化 

分 类 号：S512.9[农业科学—作物学]