16个早实核桃良种遗传多样性的FISH-AFLP分析  

FISH-AFLP Analysis of Genetic Diversity of Early-fruiting Walnut Cultivars

作　　者：马庆国[1] 齐静[1] 裴东[1] 

机构地区：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091

出　　处：《林业科学研究》2010年第5期631-636,共6页Forest Research

基　　金：“十一五”国家科技支撑计划项目(2006BAD01A1703)

摘　　要：采用FISH-AFLP技术,筛选9对E+3和M+3引物组合,对我国首批16个早实核桃良种进行了基因组DNA水平上的检测,结果表明:共获得1 072条可统计的条带,其中946条呈多态性,平均的多态性带百分率达88.12%;经9对引物检测的16个品种基因型各不相同,均得到数目不等的特征带,并能将16个早实核桃良种完全区分开。研究分析了早实核桃良种的遗传多样性和亲缘关系并建立了它们的指纹图谱。16 early-fruiting walnut（Juglans regia L.） cultivars were developed in China in 1994.These new walnut cultivars preliminarily formed an improved variety system for Chinese walnut production.The FISH-AFLP analysis was conducted in order to investigate the sibship among the 16 Chinese early-fruiting walnut cultivars.9 primer combinations were used to amplify the genomic DNAs.Total 1 072 AFLP fragments were obtained,in which 946 are polymorphic markers and the average percent of polymorphic bands is 88.12%.Different genotypes were detected and unique bands were found.The genetic diversity and their sibship among Chinese early-fruiting walnut cultivars were investigated and their fingerprintings were established in the research.

关 键 词：核桃 遗传多样性 亲缘关系 FISH-AFLP 指纹图谱 

分 类 号：S943.2[农业科学—水产养殖]