美洲黑杨PdZFR基因的克隆和分析  

Cloning and Analyzing of Gene PdZFR from Populus deltoides

作　　者：王大海[1] 苏晓华[1] 张冰玉[1] 黄秦军[1] 张香华[1] 

机构地区：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091

出　　处：《林业科学研究》2010年第5期637-643,共7页Forest Research

基　　金：引进国际先进林业科学技术”948”创新重大项目“杨树RNAi载体构建、材性相关基因克隆及功能研究”(2006-4-C01);国家973项目“速生优质杨树的聚合育种与分子改良”(No.2009CB119107)

摘　　要：根据美洲黑杨PdZFR部分cDNA序列,采用拟基因组文库步行法和RACE克隆了该基因全长cDNA及基因组DNA,并对该基因的启动子、转录起始位点、内含子分布、剪切方式、编码的氨基酸序列和该基因在染色体上的分布位置进行了分析。组织或器官特异表达分析表明:该基因与植物发育相关。The full length cDNA and genomic DNA of PdZFR were cloned from Populus deltoides by RACE and Visual Genome Library Walking methods respectively,according to its partial cDNA sequence.Meanwhile,the gene promoter,transcription initiation,intron and exon distribution,spice pattern,the amino acid sequence encoded and the location of this gene on chromosome were analyzed.The tissue or organ-specific expression analysis showed that this gene was related to plant development.

关 键 词：PdZFR 美洲黑杨 RACE VGL-Walking TATABox CAATBox 

分 类 号：S792.11[农业科学—林木遗传育种]