苏北杨树新造林地溃疡病病原菌的鉴定  

Identification on the Canker Disease of the New Poplar Plantation in North of Jiangsu Province

作　　者：郑华英[1] 钱国良[2] 徐福元[1] 胡白石[2] 张亚川[3] 解春霞[1] 许志刚[2] 

机构地区：[1]江苏省林业科学研究院,江苏南京211153 [2]南京农业大学植物保护学院,江苏南京210095 [3]江苏省淮安市森林病虫防治检疫站,江苏淮安223001

出　　处：《林业科学研究》2010年第5期649-655,共7页Forest Research

基　　金：江苏省农业三项工程项目[编号SX(2004)008-1]

摘　　要：2001年以来,苏北地区每年春季杨树新造林发生大面积死亡,在死亡或濒临死亡的杨树枝干上,几乎都有溃疡病症状的病斑发生。本试验通过对杨树新造林地(I-72杨)溃疡病病组织的分离、纯化培养获得10余株菌株,经接种、发病后再分离,获得6株拟茎点霉菌株;进一步克隆并分析供试菌株核糖体DNA-ITS区域的序列,结果是分离菌株与GenBank中Phomopsis eucommiicola的ITS序列有同源性,相似性达到99%。因此将该拟茎点霉菌确定为Phomopsis eucommiicolaC.Q.Chang,Z.D.Jiang&P.K.Chi,。该病原菌侵染杨树引起杨树新造林木溃疡病是首次报道。Every spring since 2001,large areas of the new forested poplar died in north of Jiangsu Province.In poplar branches that had died or were nearly to die,there were spots similar to the symptoms of canker disease.By isolation,inoculation and re-isolating the Koch＇s principle was completed and finally based on the sequence of ribosomal DNA-ITS,the pathogen was identified as Phomopsis eucommiicola C.Q.Chang,Z.D.Jiang P.K.Chi,This paper was the first report about Phomopsis eucommiicola causing canker disease on the new poplar forestation.

关 键 词：杨树新造林 杜仲生拟茎点霉 核糖体DNA-ITS 

分 类 号：S792.11[农业科学—林木遗传育种]