一株黄曲霉菌种对土壤氯嘧磺隆残留的降解特性  

The characteristics of Aspergillus flavus degrading chlorimuron-ethyl residues in soil

作　　者：韩婷婷[1] 孙庆元[1] 宗娟[1] 李阳[1] 

机构地区：[1]大连工业大学生物与食品工程学院,辽宁大连116034

出　　处：《大连工业大学学报》2010年第5期333-337,共5页Journal of Dalian Polytechnic University

摘　　要：为解决大豆除草剂氯嘧磺隆在土壤中残留时间长的问题,从氯嘧磺隆残留土壤中分离出一株高效降解氯嘧磺隆的真菌,经鉴定为丛梗孢目丛梗孢科曲霉属中的黄曲霉,命名为Q6。用生物测定方法测定氯嘧磺隆在土壤中的残留量,Q6在pH为6.5、温度为28℃、湿度为60%、接种量为10%、氯嘧磺隆质量分数为10μg/kg时,达到最佳降解率,在土壤中培养30 d氯嘧磺隆的降解率为94.88%。在氯嘧磺隆残留土壤中施用Q6的油菜出苗率由10%提高到80%,35 d后,油菜的株高和鲜重均达到对照的90%左右,不施用Q6的只有对照的45%左右,结果表明Q6能高效降解土壤中残留的氯嘧磺隆,对被氯嘧磺隆毒害的土壤有很好的修复作用。In order to solve the problem of soybean herbicide chlorimuron-ethyl residues in soil for too long time, one strain degrading soybean herbicide chlorimuron-ethyl was isolated and identified as Aspergillus flavus, which named Q6. The residue degradation of the chlorimuron ethyl in the soil was analyzed and the optimum condition was as follows： pH 6.5, temperature 28 ℃, humidity 60%, inoculum 10 % and the concentration of chlorimuron-ethyl 10μg/kg, in which the degradation rate can reached to 94.88% after 30 days. Rape plant could grow normally in the soil with chlorimuron-ethyl and its emergence rate was increased from 10% to 80% when Q6 was used. The height and fresh weight reached to about 90% with Q6 but only 45% of the control was obtained without Q6. The results showed that Q6 could efficiently degrade the residue of chlorimuron-ethyl e.xhibiting a prothetic effect on the contaminated soil.

关 键 词：氯嘧磺隆 生物降解 氯仿熏蒸法 

分 类 号：Q93-335[生物学—微生物学]