采用BS-Ⅱ大孔离子交换树脂分离低分子肝素  

Purification of low molecular weight heparin by macroporous ion exchange resin BS-Ⅱ

作　　者：李磊[1] 温馨蔚[1] 雷昊[1] 唐万燕[1] 余蓉[1] 

机构地区：[1]四川大学华西药学院,成都610041

出　　处：《生物加工过程》2010年第5期48-52,共5页Chinese Journal of Bioprocess Engineering

基　　金：科技部创新基金资助项目(06C26215100476)

摘　　要：采用HNO2降解法,通过控制降解过程中的pH和时间裂解低分子肝素,并经大孔离子交换树脂BS-Ⅱ对其进行分离纯化,用质谱法对其相对分子质量进行分析。结果表明：在pH3.5、4 h的降解条件下,降解得到低分子肝素。在0.34 mol/L NaCl、pH8.5的条件下吸附,4 mol/L NaCl、pH8.5条件下洗脱,其吸附率达97.2%,纯化后的低分子肝素抗凝活性为34.41 U/mg,比纯化前提高1.36倍,效价回收率达到85.24%。质谱分析表明,纯化所得低分子肝素的平均相对分子质量为8 411,67.45%集中分布在7 000-9 000段。A method for preparing low molecular weight heparin（LMWH） by depolymerization with nitrous acid was studied.Factors pH and time had been investigated during the process.Depolymerized heparin was purified by resin BS-Ⅱ and analyzed by mass spectrometry.LMWH was prepared under the operating conditions of pH 3.5 and reaction time of 4 h.The adsorption condition was 0.34 mol/L NaCl at pH 8.5 and the elution condition was 4 mol/L NaCl at pH 8.5.The adsorption rate of of the depolymerized heparin was 97.2%;the anticoagulant activity of the purified LMWH was 34.41U/mg,thus it increased 1.36 times higher than pre-purified,and the recovery activity rate was 85.24%.The result of MS showed that average molecular weight of the purified LMWH was 8 411,and 67.45% of which distributed among the molecular weights of 7 000-9 000.

关 键 词：低分子肝素 大孔离子交换树脂 分离纯化 

分 类 号：TQ464[化学工程—制药化工]